DNA methylation abnormalities in congenital heart disease
| Autor: | Luis A. Pérez-Jurado, Nuria Toran, Aïda Homs, Raquel Flores, Ivon Cuscó, Clara Serra-Juhé |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Heart Defects Congenital Male Cancer Research Candidate gene Down syndrome Pathology medicine.medical_specialty Heart disease Heart malformation ADN Biology Bioinformatics Malalties coronàries Young Adult Fetus Pregnancy medicine Humans Epimutation Epigenetics Molecular Biology Aged Congenital heart disease Aged 80 and over DNA methylation Myocardium DNA Methylation Middle Aged medicine.disease Outflow tract morphogenesis Female Genome-Wide Association Study Research Paper |
| Zdroj: | Recercat. Dipósit de la Recerca de Catalunya instname Epigenetics |
| Popis: | Congenital heart defects represent the most common malformation at birth, occurring also in ∼50% of individuals with Down syndrome. Congenital heart defects are thought to have multifactorial etiology, but the main causes are largely unknown. We have explored the global methylation profile of fetal heart DNA in comparison to blood DNA from control subjects: an absolute correlation with the type of tissue was detected. Pathway analysis revealed a significant enrichment of differential methylation at genes related to muscle contraction and cardiomyopathies in the developing heart DNA. We have also searched for abnormal methylation profiles on developing heart-tissue DNA of syndromic and non-syndromic congenital heart defects. On average, 3 regions with aberrant methylation were detected per sample and 18 regions were found differentially methylated between groups. Several epimutations were detected in candidate genes involved in growth regulation, apoptosis and folate pathway. A likely pathogenic hypermethylation of several intragenic sites at the MSX1 gene, involved in outflow tract morphogenesis, was found in a fetus with isolated heart malformation. In addition, hypermethylation of the GATA4 gene was present in fetuses with Down syndrome with or without congenital heart defects, as well as in fetuses with isolated heart malformations. Expression deregulation of the abnormally methylated genes was detected. Our data indicate that epigenetic alterations of relevant genes are present in developing heart DNA in fetuses with both isolated and syndromic heart malformations. These epimutations likely contribute to the pathogenesis of the malformation by cis-acting effects on gene expression. This work was supported by grants of the Spanish Fondo de Investigación Sanitaria of the Ministry of Economy and Competitiveness and FEDER funds [FIS PI10/2512 and PI13/2812], an intramural project of the CIBERER, the Catalan Government [SGR2009/1274, SGR2014/1468 and ICREA Acadèmia] and a predoctoral fellowship of the Fondo de Investigación Sanitaria [FIS FI08/00365] to CS-J. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|