Glutamine as a modulator of the immune system of critical care patients: Effect on Toll-like receptor expression. A preliminary study
| Autor: | Alberto Rodríguez, Verónica Regueiro, José A. Bengoechea, Jordi Ibáñez, Pedro Marsé, Abelardo García de Lorenzo Mateos, Joan Maria Raurich, Jon Pérez-Bárcena |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Parenteral Nutrition medicine.medical_specialty Adolescent Critical Care Critical Illness Glutamine Endocrinology Diabetes and Metabolism CD14 Lipopolysaccharide Receptors Bacteremia Monocytes Flow cytometry Basal (phylogenetics) Immune system Sepsis Internal medicine medicine Humans Single-Blind Method Prospective Studies Receptor Prospective cohort study Aged Nutrition and Dietetics medicine.diagnostic_test business.industry Pneumonia Middle Aged Flow Cytometry Toll-Like Receptor 2 Toll-Like Receptor 4 Endocrinology Parenteral nutrition Gene Expression Regulation Female business |
| Zdroj: | Nutrition. 24:522-527 |
| ISSN: | 0899-9007 |
| DOI: | 10.1016/j.nut.2008.01.056 |
| Popis: | Objective We evaluated the expression of Toll-like receptors 2 and 4 (TLR-2 and TLR-4) in circulating monocytes from peripheral blood of critical care patients treated with and without glutamine. Because no research has been published to date on the effect of glutamine on TLR receptors in critical patients, it was determined in an initial sample of 30 patients. Methods This was a prospective, randomized, single-blind study with 15 patients assigned to receive parenteral nutrition with a daily glutamine supplement of 0.35 g/kg. The control group received isocaloric-isonitrogenous parenteral nutrition. Blood samples were extracted before beginning the treatment and at 5 and 14 d. Expressions of CD14, TLR-2, and TLR-4 were determined by flow cytometry. Levels of TLRs were expressed as mean fluorescence intensity (mfi). Results Basal characteristics were similar in both groups. The expressions of TLR-2 in the treatment group with glutamine were 4.67 ± 3.82 mfi before treatment, 3.91 ± 2.04 mfi at 5 d, and 4.28 ± 2.47 mfi at 14 d. The expressions of TLR-2 in the control group were 5.49 ± 3.20 mfi before treatment, 4.48 ± 2.15 mfi at 5 d, and 4.36 ± 2.36 mfi at 14 d. The expressions of TLR-4 in the treatment group were 1.65 ± 1.89 mfi before treatment, 1.23 ± 1.10 mfi at 5 d, and 1.77 ± 1.97 at 14 d. The expressions of TLR-4 in the control group were 1.51 ± 1.76 mfi before treatment, 1.36 ± 0.99 mfi at 5 d, and 1.26 ± 0.59 mfi at 14 d. Infections were detected in 11 patients who received glutamine and 13 control patients (P = 0.51). Conclusion In critical care patients, parenteral nutrition supplemented with glutamine does not increase the expression of TLR-2 or TLR-4 in peripheral blood monocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect