FABP5 as a possible biomarker in atopic march: FABP5-induced Th17 polarization, both in mouse model and human samples

Autor: Ji Hye Kim, Jung Won Park, Thomas S. Kupper, Jungsoo Lee, Ke Lun Zhang, Youdong Pan, Kyung Hee Park, Seo Hyeong Kim, Jongsun Lee, Ji Yeon Noh, HyeRan Kim, Chang Ook Park, Bomi Kim, Kyoung Yong Jeong, Kwang Hoon Lee, Howard Chu, Zheng-wang Sun
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
CD4-Positive T-Lymphocytes
Male
Research paper
AHR
Airway hyperresponsiveness
medicine.medical_treatment
Th1
Type 1 helper T cells
lcsh:Medicine
AA
Allergic asthma
medicine.disease_cause
AR
Allergic rhinitis
Mice
0302 clinical medicine
Allergen
EASI
Eczema area and severity index
IL-17A
TRC
The RNAi Consortium
D. farinae
Dermatophagoides farinae
TSLP
Thymic stromal lymphopoietin
Sensitization
Cells
Cultured
Oligonucleotide Array Sequence Analysis
lcsh:R5-920
medicine.diagnostic_test
Cell Polarity
Fatty-acid-binding protein 5
General Medicine
Atopic dermatitis
Middle Aged
Up-Regulation
Real-time polymerase chain reaction
Cytokine
medicine.anatomical_structure
ELISA
Enzyme-linked immunosorbent assay
030220 oncology & carcinogenesis
Disease Progression
AD
Atopic dermatitis
Biomarker (medicine)
Female
TRM
Tissue-resident memory T
Th17
lcsh:Medicine (General)
HC
Healthy controls
Atopic march
Adult
Genetic Markers
Thymic stromal lymphopoietin
Fatty Acid-Binding Proteins
General Biochemistry
Genetics and Molecular Biology
Flow cytometry
Dermatitis
Atopic
shRNA
Short-hairpin ribonucleic acid
03 medical and health sciences
Young Adult
AM
Atopic march
medicine
Animals
Humans
Antigens
Dermatophagoides
HDM
House dust mite
FABP
Fatty acid-binding protein
E-FABP
Epidermal fatty acid-binding protein
KO
knockout
business.industry
Th2
Type 2 helper T cells
lcsh:R
Dendritic Cells
SDS
Sodium dodecyl sulfate
medicine.disease
ROR
Retinoic acid-related orphan receptor
Coculture Techniques
Disease Models
Animal
FABP5L
Fatty acid-binding protein5-like
030104 developmental biology
Case-Control Studies
Immunology
Th17
Type 17 helper T cells
Th17 Cells
Der f 1
Dermatophagoides farinae 1
business
qRT-PCR
Quantitative real-time polymerase chain reaction
Zdroj: EBioMedicine, Vol 58, Iss, Pp 102879-(2020)
EBioMedicine
ISSN: 2352-3964
Popis: Background While the incidence of patients with atopic dermatitis (AD) with atopic march (AM) showing respiratory allergy is steadily rising, the pathomechanism is still unknown. There are currently no biomarkers to predict progression of AM. Methods To explore the mechanism of AM, patients with AD and AM and healthy controls were recruited and RNA microarray, flow cytometry, quantitative real-time polymerase chain reaction, and immunofluorescence staining were performed. We also co-cultured dendritic cells and CD4+ T cells with various Dermatophagoides farinae allergen fractions. Cytokine levels were evaluated using enzyme-linked immunosorbent assay. Findings Both fatty-acid-binding protein 5 (FABP5) and Th17-related genes were more highly expressed in AM. FABP5 knockdown significantly decreased Th17-inducing cytokines in keratinocytes and IL-17A in T cells from AM patients. Further confirmation was obtained using an AM mice model compared to mice without AM. Der f 1, a major D. farinae allergen, increased FABP5 and IL-17A expression in T cells from AM patients. Higher serum FABP5 levels from AM patients were positively correlated with serum IL-17A levels. Interpretation FABP5 expression, possibly enhanced by higher epicutaneous and respiratory sensitization to Der f 1, may directly promote Th17 responses in AD patients with AM. Thus, AM progression can be explained by Th17 reaction induced by FABP5. FABP5 was identified as a potential biomarker in AM. Funding This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Science and ICT; No. NRF-2017R1A2B4009568), grants of the Korean Health Technology R&D Project, Ministry for Health, Welfare & Family Affairs, and the Republic of Korea (HI13C0010, HI14C1324, HI14C1799).
Databáze: OpenAIRE
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