Integrated omics in Drosophila uncover a circadian kinome
| Autor: | Bo Wen, Hui Hu, Ying Zhang, Hao-Dong Xu, Shanshan Ma, Ke Shui, Shaofeng Lin, Wankun Deng, Yu Xue, An-Yuan Guo, Chenwei Wang, Luoying Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Proteomics
0301 basic medicine Science Circadian clock Regulator Gene regulatory network General Physics and Astronomy Endogeny Computational biology Biology Article General Biochemistry Genetics and Molecular Biology Substrate Specificity 03 medical and health sciences 0302 clinical medicine Tandem Mass Spectrometry Genetics Locomotor rhythm Animals Drosophila Proteins Gene Regulatory Networks Kinome Circadian rhythm Phosphorylation lcsh:Science Multidisciplinary Gene Expression Profiling Phosphotransferases Phosphoproteomics General Chemistry Phosphoproteins Circadian Rhythm Computational biology and bioinformatics Drosophila melanogaster 030104 developmental biology lcsh:Q Systems biology Algorithms 030217 neurology & neurosurgery Chromatography Liquid |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-16514-z |
| Popis: | Most organisms on the earth exhibit circadian rhythms in behavior and physiology, which are driven by endogenous clocks. Phosphorylation plays a central role in timing the clock, but how this contributes to overt rhythms is unclear. Here we conduct phosphoproteomics in conjunction with transcriptomic and proteomic profiling using fly heads. By developing a pipeline for integrating multi-omics data, we identify 789 (~17%) phosphorylation sites with circadian oscillations. We predict 27 potential circadian kinases to participate in phosphorylating these sites, including 7 previously known to function in the clock. We screen the remaining 20 kinases for effects on circadian rhythms and find an additional 3 to be involved in regulating locomotor rhythm. We re-construct a signal web that includes the 10 circadian kinases and identify GASKET as a potentially important regulator. Taken together, we uncover a circadian kinome that potentially shapes the temporal pattern of the entire circadian molecular landscapes. Phosphorylation plays an important role in the regulation of molecular circadian clocks. Here the authors utilize multi-omics data from flies to describe the circadian kinome and identify GASKET as a potentially important regulator within the circadian kinase network. |
| Databáze: | OpenAIRE |
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