Isolation and Expression of a cDNA Encoding the Precursor for a Novel Member (ACADSB) of the Acyl-CoA Dehydrogenase Gene Family
| Autor: | Caroline Vicanek, Rima Rozen, Katherine Fu, Beatrice Fournier, Elena Torban, Jan Willard, Renate Milos, Lawrence Low-Nang, Jerry Vockley, Lubing Zhou |
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| Rok vydání: | 1994 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Acyl-CoA Dehydrogenase Homology (biology) Cell Line Substrate Specificity ACADSB Acyl-CoA Dehydrogenases Complementary DNA Genetics Animals Humans Gene family Amino Acid Sequence Peptide sequence DNA Primers chemistry.chemical_classification Enzyme Precursors Base Sequence biology Acyl CoA dehydrogenase Molecular biology Amino acid Open reading frame chemistry Biochemistry biology.protein |
| Zdroj: | Genomics. 24:280-287 |
| ISSN: | 0888-7543 |
| DOI: | 10.1006/geno.1994.1617 |
| Popis: | The acyl-CoA dehydrogenases (ACDs) are a family of mitochondrial enzymes that oxidize straight chain or branched chain acyl-CoAs in the metabolism of fatty acids or branched chain amino acids. Deficiencies in members of this gene family are important causes of human disease. A cDNA encoding the human precursor for a novel member (gene symbol ACADSB) of the ACD gene family has been isolated and characterized. The open reading frame of 1.3 kb encodes a precursor protein of 431 amino acids, which is processed in vitro to yield a mature protein of 399 amino acids. The cDNA has significant sequence similarity to other members of the acyl-CoA dehydrogenase family, with the greatest homology (38%) to the short chain acyl-CoA dehydrogenase. The cDNA was expressed in eukaryotic (COS) and prokaryotic (Escherichia coli) cells, producing a protein of the expected size, with activity toward the short branched chain acyl-CoA derivatives ((S)-2-methylbutyryl-CoA, isobutyryl-CoA, and 2-methylhexanoyl-CoA), as well as toward the short straight chain acyl-CoAs (butyryl-CoA and hexanoyl-CoA). |
| Databáze: | OpenAIRE |
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