Generation of red drum (Sciaenops ocellatus) hematology Reference Intervals with a focus on identified outliers
| Autor: | Ryan Takeshita, Steven A. Murawski, Kendal E. Harr, Kristina Deak, Drury R. Reavill |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Veterinary medicine Fish species 010501 environmental sciences Biology 01 natural sciences 03 medical and health sciences Reference Values Internal medicine medicine Animals Petroleum Pollution 0105 earth and related environmental sciences Hematology General Veterinary Fishes Baseline data Crude oil Reference intervals 030104 developmental biology Deepwater horizon Oil spill Fish Female |
| Zdroj: | Veterinary Clinical Pathology. 47:22-28 |
| ISSN: | 0275-6382 |
| Popis: | BACKGROUND The Deepwater Horizon (DWH) oil spill in 2010 released millions of barrels of crude oil into the northern Gulf of Mexico, exposing numerous species of animals to the toxic components of oil. A comprehensive assessment of morbidity and mortality caused by DWH oil exposure was undertaken by the DWH Natural Resource Damage Trustees to characterize ecosystem damages. OBJECTIVES This study aimed to characterize normal hematologic RIs in red drum fish with blood cell descriptions, and to demonstrate the importance of identifying and removing outliers when generating RI. METHODS Two years after the oil spill, 57 adult, red drum fish of mixed sexes were caught along the eastern Louisiana coastline. Eight different sites were chosen to catch the fish; 6 sites were contaminated with oil, and 2 sites were not contaminated at the time of the oil spill. Hematologic RIs were generated from heparinized whole blood samples of healthy red drum as determined by gross examination and histopathologic examination. Two methods were used to detect hematologic effects likely caused by oil contamination. RESULTS Red drum PCVs (RI 42-62%) were higher than previously reported in cold water and bottom-dwelling fish species, while absolute WBC counts (RI 2.9-8.7 × 109 /L) were comparable to WBC counts previously reported in other fish species with heterophil and lymphocyte absolute concentrations frequently being equivalent. Anemic animals (PCV |
| Databáze: | OpenAIRE |
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