Efficacy of Hospital Cleaning Agents and Germicides Against EpidemicClostridium difficileStrains
| Autor: | Simon D. Baines, Katie Saxton, Jane Freeman, Keith Stephenson, Robert C. Owens, Warren N. Fawley, Mark H. Wilcox, Sarah Underwood |
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| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
Cleaning agent Sodium Hypochlorite Epidemiology Detergents Population Hypochlorite Microbial Sensitivity Tests Microbiology chemistry.chemical_compound Humans Clostridiaceae Hydrogen peroxide education Spores Bacterial Cross Infection education.field_of_study biology Clostridioides difficile Hydrogen Peroxide Clostridium difficile biology.organism_classification Antimicrobial Anti-Bacterial Agents Spore Infectious Diseases chemistry |
| Zdroj: | Infection Control & Hospital Epidemiology. 28:920-925 |
| ISSN: | 1559-6834 0899-823X 0195-9417 |
| DOI: | 10.1086/519201 |
| Popis: | Objective.To compare the effects of hospital cleaning agents and germicides on the survival of epidemicClostridium difficilestrains.Methods.We compared the activity of and effects of exposure to 5 cleaning agents and/or germicides (3 containing chlorine, 1 containing only detergent, and 1 containing hydrogen peroxide) on vegetative and spore forms of epidemic and non-epidemicC. difficilestrains (3 of each). We carried out in vitro exposure experiments using a human fecal emulsion to mimic conditions found in situ.Results.Cleaning agent and germicide exposure experiments yielded very different results forC. difficilevegetative cells, compared with those for spores. Working-strength concentrations of all of the agents inhibited the growth ofC. difficilein culture. However, when used at recommended working concentrations, only chlorine-based germicides were able to inactivateC. difficilespores.C. difficileepidemic strains had a greater sporulation rate than nonepidemic strains. The mean sporulation rate, expressed as the proportion of a cell population that is in spore form, was 13% for all strains not exposed to any cleaning agent or germicide, and it was significantly increased by exposure to cleaning agents or germicides containing detergent alone (34%), a combination of detergent and hypochlorite (24%), or hydrogen peroxide (33%). By contrast, the mean sporulation rate did not change substantially after exposure to germicides containing either a combination of detergent and dichloroisocyanurate (9%) or dichloroisocyanurate alone (15%).Conclusions.These results highlight differences in the activity of cleaning agents and germicides againstC. difficilespores and the potential for some of these products to promote sporulation. |
| Databáze: | OpenAIRE |
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