Evaluation of the structure of the otoconial layer using micro-computed tomography
| Autor: | Takeshi Kiyoi, Yuuki Imai, Masachika Shudou, Eriko Nishihara, Naohito Hato, Masahiro Okada |
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| Rok vydání: | 2019 |
| Předmět: |
Ovariectomy
Sham group 03 medical and health sciences Mice Otolithic Membrane 0302 clinical medicine Bone Density Utricle Medicine Animals 030223 otorhinolaryngology business.industry Micro computed tomography Uterus Sham surgery General Medicine Anatomy X-Ray Microtomography Mice Inbred C57BL surgical procedures operative medicine.anatomical_structure Otorhinolaryngology 030220 oncology & carcinogenesis Models Animal Surgery Female sense organs business hormones hormone substitutes and hormone antagonists |
| Zdroj: | Auris, nasus, larynx. 47(5) |
| ISSN: | 1879-1476 |
| Popis: | Estrogen deficiency caused by bilateral ovariectomy (OVX) has been reported to lead to morphological changes in otoconia. Thus, we examined the morphological changes in the otoconial layer after OVX. We also investigated whether micro-computed tomography (µCT) is useful for the detection of morphological changes in the otoconial layer.The otic capsules of C57BL/6 J mice were removed and evaluated using histological techniques and µCT at 2, 4, and 8 weeks after OVX or sham surgery. The volume of the utricle otoconial layer was measured and compared between the OVX and sham groups. The µCT scan and histological study results were also compared.The volume of the utricle otoconial layer was significantly increased 4 weeks after OVX compared to the sham group in both histological and µCT studies (p 0.05). The volume of the otoconial layer measured using µCT was significantly correlated with the histological study results (p 0.05).The volume of the utricle otoconial layer increased after OVX. These morphological changes could be detected by µCT. |
| Databáze: | OpenAIRE |
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