Immunochemical detection of human blood in feces
| Autor: | Hirofumi Miyoshi, Ichiro Hirata, Seiji Tsumoto, Ryoichi Matsuse, Saburo Ohshiba, Norimitsu Miyachi, Kazuo Uchida, Shouji Okuda, Shinobu Tomita |
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| Rok vydání: | 1990 |
| Předmět: |
Erythrocytes
Colorectal cancer Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Biology Biochemistry Immunoenzyme Techniques Feces Hemoglobins Plasma Antibody Specificity medicine Animals Humans chemistry.chemical_classification Human blood Biochemistry (medical) Fecal occult blood Transferrin Antibodies Monoclonal General Medicine medicine.disease Molecular biology Colon polyps chemistry Occult Blood Cattle Hemoglobin Quantitative analysis (chemistry) |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 189(3) |
| ISSN: | 0009-8981 |
| Popis: | We have developed a new immunochemical test for fecal occult blood utilizing enzyme-linked immunosorbent assay (ELISA) of human hemoglobin (HbAo) and transferrin (Tf) simultaneously. The ELISA had a sensitivity of about 15 ng/ml Hb, and the measurable range was 1.5-750 micrograms Hb per g feces. The stability of Tf in feces was greater than that of Hb. In 17 out of 18 patients with colon cancer, 8 out of 15 patients with colon polyps, and 11 out of 20 patients with upper-gastrointestinal disorders. The Hb and Tf values were more than 10 micrograms/g feces, in terms of Hb concentration. The ELISA for human fecal HbAo and Tf might be useful for the diagnosis of gastrointestinal disorders. |
| Databáze: | OpenAIRE |
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