Facilitation of trace metal uptake in cells by inulin coating of metallic nanoparticles
| Autor: | Bryan J. Cole, Bing Wu, Fernando Arteaga-Cardona, Gary N. Cherr, Miguel A. Méndez-Rojas, Cristina Torres-Duarte, Esmeralda Santillán-Urquiza |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
inulin coating
food fortification Inulin chemistry.chemical_element zinc oxide nanoparticles Bioengineering 02 engineering and technology Zinc 010501 environmental sciences 01 natural sciences chemistry.chemical_compound Cellular and Molecular Biology Nanotechnology Trace metal Viability assay lcsh:Science 0105 earth and related environmental sciences Nutrition chemistry.chemical_classification Reactive oxygen species Multidisciplinary 021001 nanoscience & nanotechnology Bioavailability chemistry Biochemistry Nanotoxicology nanotoxicity Toxicity mussel haemocytes Biophysics lcsh:Q Generic health relevance 0210 nano-technology Biotechnology Research Article |
| Zdroj: | Royal Society Open Science, Vol 4, Iss 9 (2017) Royal Society Open Science Royal Society open science, vol 4, iss 9 |
| ISSN: | 2054-5703 |
| DOI: | 10.1098/rsos.170480 |
| Popis: | Trace elements such as zinc and iron are essential for the proper function of biochemical processes, and their uptake and bioavailability are dependent on their chemical form. Supplementation of trace metals through nanostructured materials is a new field, but its application raises concerns regarding their toxicity. Here, we compared the intracellular zinc uptake of different sources of zinc: zinc sulfate, and ZnO and core-shell α-Fe 2 O 3 @ZnO nanoparticles, coated or uncoated with inulin, an edible and biocompatible polysaccharide. Using mussel haemocytes, a well-known model system to assess nanomaterial toxicity, we simultaneously assessed zinc accumulation and multiple cellular response endpoints. We found that intracellular zinc uptake was strongly enhanced by inulin coating, in comparison to the uncoated nanoparticles, while no significant effects on cell death, cell viability, mitochondrial membrane integrity, production of reactive oxygen species or lysosome abundance were observed at concentrations up to 20 ppm. Since no significant increments in toxicity were observed, the coated nanomaterials may be useful to increase in vivo zinc uptake for nutritional applications. |
| Databáze: | OpenAIRE |
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