Human mesenchymal adipose stromal cells from mature adipocyte fraction
| Autor: | Ausra Unguryte, Algirdas Venalis, Eiva Bernotiene |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Stromal cell
QH301-705.5 Adipose tissue macrophages Population Adipose tissue Biology General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Adipocyte Biology (General) education adipose education.field_of_study General Immunology and Microbiology General Neuroscience Mesenchymal stem cell 3T3-L1 differentiation Stromal vascular fraction multipotent stem cells Cell biology chemistry Immunology mesenchymal stromal cells General Agricultural and Biological Sciences ceiling culture |
| Zdroj: | Open Life Sciences, Vol 5, Iss 1, Pp 47-58 (2010) |
| ISSN: | 2391-5412 |
| DOI: | 10.2478/s11535-009-0073-6 |
| Popis: | More effective techniques should be employed for isolation of human mesenchymal stromal cells derived from adipose tissue (ADSC), seeking to make adipose tissue biopsies smaller in volume and thus less invasive. In this study, we compared properties of ADSC isolated by several different methods from the same samples of adipose tissue in order to enhance yields of potential ADSC. The mature adipocyte fraction was investigated using the ceiling culture method, including both ceiling and bottom cell fractions, and the control culture method with standard amount of medium. The results were also compared using the stromal vascular fraction from the same samples. The most efficient was the bottom cell population isolated from the mature adipocyte fraction by ceiling culture method. These cells readily differentiated into osteogenic, adipogenic and chondrogenic lineages and, similar to stromal vascular fraction cells, displayed high proliferation potential. Cultures of mature adipocyte fractions with standard amount of medium were considerably less effective. Mature adipocyte fractions yields large quantities of adipose-derived stem cells that have properties comparable with stromal vascular fraction cells suitable for tissue regeneration, especially when only small biopsies can be taken. |
| Databáze: | OpenAIRE |
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