Optimization and validation of the methods for the total mercury and methylmercury determination in breast milk
| Autor: | Marion Hulin, Jean-Luc Volatier, Alexandra Rousseau, Jean Dumont, Véronique Sirot, Justine Verdeil, Thierry Guérin, R Serreau, Rachida Chekri, Tabassome Simon, Fabienne Séby, Véronique Vacchina |
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| Přispěvatelé: | Centre de Ressources Biologiques HUEP-UPMC (CRB HUEP-UPMC), UMS omique (OMIQUE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU) |
| Rok vydání: | 2017 |
| Předmět: |
[SDV]Life Sciences [q-bio]
chemistry.chemical_element Food Contamination 010501 environmental sciences Isotope dilution Breast milk 01 natural sciences High-performance liquid chromatography Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Dry weight Limit of Detection Humans Methylmercury Inductively coupled plasma mass spectrometry ComputingMilieux_MISCELLANEOUS Chromatography High Pressure Liquid 0105 earth and related environmental sciences Chromatography Milk Human 010401 analytical chemistry Mercury Repeatability Methylmercury Compounds 0104 chemical sciences Mercury (element) chemistry Environmental chemistry Linear Models Food Analysis |
| Zdroj: | Talenta Talenta, 2017 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2017.02.046 |
| Popis: | The objective of the work was to develop and validate methods for the total Hg and methylmercury (MeHg) in breast milk that could be further used to obtain first data on chemical contamination of French breast milk. For total Hg determination, the potential of two techniques, namely Advanced Mercury Analyzer (AMA) and ICP MS, was compared. For MeHg determination, ICP MS detection associated to a quantification by isotopic dilution was used and the potential of a preliminary separation by gas or liquid chromatography was evaluated and discussed. The optimization studies have shown that AMA for total Hg determination and HPLC - ID - ICP MS, after a preconcentration step by freeze-drying, for MeHg quantification were the most relevant methods to use for epidemiologic studies. The figures of merit for both methods were evaluated by means of accuracy profiles in terms of limits of quantification (1.82 and 1.35µg Hg/kg dry weight, corresponding to 0.22 and 0.16µg Hg/kg wet basis for total Hg and MeHg, respectively), repeatability (2-11% and 3-8% for total Hg and MeHg respectively), intermediate precision reproducibility (4-12% and 4-8% for total Hg and MeHg respectively) and trueness bias (-0.1-9% and -4-0% for total Hg and MeHg respectively). The methods were then applied to 180 breast milk samples. Total Hg concentrations ranged from |
| Databáze: | OpenAIRE |
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