Dietary sunflower oil modulates milk fatty acid composition without major changes in adipose and mammary tissue fatty acid profile or related gene mRNA abundance in sheep

Autor: Christine Leroux, Gonzalo Hervás, Laurence Bernard, Pilar Frutos, Álvaro Belenguer, T. Castro-Carrera, Yves Chilliard, Pablo G. Toral
Přispěvatelé: Ministerio de Economía y Competitividad (España), Junta de Castilla y León, Institut National de la Recherche Agronomique (France), European Commission, Instituto de Ganadería de Montana (IGM), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement
Jazyk: angličtina
Rok vydání: 2015
Předmět:
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
CD36
Adipose tissue
Lipogenic gene expression
0403 veterinary science
Gene expression
Sunflower Oil
Adiposity
2. Zero hunger
chemistry.chemical_classification
ACACA
biology
Fatty Acids
04 agricultural and veterinary sciences
fatty acid composition
lactating ewe
lipogenic gene expression
plant oil
tissue
Plant oil
Animal culture
Milk
Adipose Tissue
Biochemistry
Female
Polyunsaturated fatty acid
medicine.medical_specialty
food.ingredient
040301 veterinary sciences
SF1-1100
food
Dietary Fats
Unsaturated
Internal medicine
medicine
Animals
Lactation
Plant Oils
RNA
Messenger
Sheep
Tissue
Sunflower oil
0402 animal and dairy science
Fatty acid
Lipid metabolism
Lipid Metabolism
040201 dairy & animal science
Endocrinology
Lactating ewe
chemistry
Dietary Supplements
biology.protein
Animal Science and Zoology
Fatty acid composition
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Zdroj: Animal, Vol 9, Iss 4, Pp 582-591 (2015)
animal
animal, Published by Elsevier (since 2021) / Cambridge University Press (until 2020), 2015, 9 (4), pp.582-591. ⟨10.1017/S1751731114002882⟩
Animal
Animal, Published by Elsevier (since 2021) / Cambridge University Press (until 2020), 2015, 9 (4), pp.582-591. ⟨10.1017/S1751731114002882⟩
Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 1751-7311
1751-732X
Popis: Published online: 02 December 2014.--10 páginas, 7 tablas.
There are very few studies in ruminants characterizing mammary and adipose tissue (AT) expression of genes and gene networks for diets causing variations in milk fatty acid (FA) composition without altering milk fat secretion, and even less complementing this information with data on tissue FA profiles. This work was conducted in sheep to investigate the response of the mammary gland and the subcutaneous and perirenal AT, in terms of FA profile and mRNA abundance of genes involved in lipid metabolism, to a diet known to modify milk FA composition. Ten lactating Assaf ewes were randomly assigned to 2 treatments consisting of a total mixed ration based on alfalfa hay and a concentrate (60:40) supplemented with 0 (control diet) or 25 (SO diet) g of sunflower oil/kg of diet DM for 7 weeks. Milk composition, including FA profile, was analysed after 48 days on treatments. On day 49, the animals were euthanized and tissue samples were collected to analyse FA and mRNA abundance of 16 candidate genes. Feeding SO did not affect animal performance but modified milk FA composition. Major changes included decreases in the concentration of FA derived from de novo synthesis (e.g., 12:0, 14:0 and 16:0) and increases in that of long chain FA (e.g., 18:0, c9-18:1, trans-18:1 isomers and c9,t11-CLA) but they were not accompanied by significant variations in the mRNA abundance of studied lipogenic genes (i.e., ACACA, FASN, LPL, CD36, FABP3, SCD1 and SCD5) and transcription factors (SREBF1 and PPARG), or in the constituent FA of mammary tissue. Regarding the FA composition of AT, the little influence of SO did not appear to be linked to changes in gene mRNA abundance (decreases of GPAM and SREBF1 in both tissues, and of PPARG in the subcutaneous depot). Similarly, the great variation between AT (higher contents of saturated FA and trans-18:1 isomers in the perirenal, and of cis-18:1, c9,t11-CLA and n-3 PUFA in the subcutaneous AT) could not be related to differences in gene mRNA abundance due to tissue site (higher LPL and CD36, and lower SREBF1 in perirenal than in subcutaneous AT). Overall, these results suggest a marginal contribution of gene expression to the nutritional regulation of lipid metabolism in these tissues, at least with the examined diets and after 7 weeks on treatments. It cannot be ruled out, however, that the response to SO is mediated by other genes or post-transcriptional mechanisms.
This study was supported by the Spanish Ministry of Economy and Competitiveness (AGL2011-23700), the Council of Castile and Leon (CSI023U13), and the INRA (UMR1213; France). T. Castro-Carrera was granted a predoctoral fellowship from the Spanish National Research Council (CSIC; JAE Programme) supported by European Social Fund.
Databáze: OpenAIRE
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