A novel heterodimeric cysteine protease is required for interleukin-1βprocessing in monocytes

Autor:	John A. Schmidt, Matthew J. Kostura, Anna M. Rolando, Richard A. Mumford, Ting-Ting Yamin, Guadalupe A. Limjuco, Keith O. Elliston, Jeffrey R. Weidner, J. Paul Salley, Jayne Chin, Susan M. Molineaux, S.M. Raju, John G. Aunins, Gloria J.-F. Ding, Douglas K. Miller, John E. Shively, Oksana C. Palyha, Julia M. Ayala, Nancy A. Thornberry, Michael J. Tocci, Malcolm MacCross, Francesca J. Casano, Herbert G. Bull, Andrew D. Howard, Linda A. Egger, Kevin T. Chapman, Jimmy R. Calaycay, Erin P. Gaffney, Terry D. Lee
Rok vydání:	1992
Předmět:	Molecular Sequence Data Caspase 1 Peptide Molecular cloning Biology Binding Competitive Chromatography Affinity Chromatography DEAE-Cellulose Monocytes Substrate Specificity Open Reading Frames Sequence Homology Nucleic Acid Complementary DNA Humans Amino Acid Sequence Cloning Molecular Peptide sequence Chromatography High Pressure Liquid chemistry.chemical_classification Multidisciplinary Base Sequence Caspase Gene Chromosome Mapping Metalloendopeptidases Cysteine protease Enzyme Diazomethane chemistry Biochemistry Electrophoresis Polyacrylamide Gel Protein Processing Post-Translational Interleukin-1
Zdroj:	Nature. 356:768-774
ISSN:	1476-4687 0028-0836
DOI:	10.1038/356768a0
Popis:	Interleukin-1 beta (IL-1 beta)-converting enzyme cleaves the IL-1 beta precursor to mature IL-1 beta, an important mediator of inflammation. The identification of the enzyme as a unique cysteine protease and the design of potent peptide aldehyde inhibitors are described. Purification and cloning of the complementary DNA indicates that IL-1 beta-converting enzyme is composed of two nonidentical subunits that are derived from a single proenzyme, possibly by autoproteolysis. Selective inhibition of the enzyme in human blood monocytes blocks production of mature IL-1 beta, indicating that it is a potential therapeutic target.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e08915c48c8d1641db36838593e5c7a0 https://doi.org/10.1038/356768a0 Zobrazit plný text záznamu