Antibacterial Properties of Chitosan Nanoparticles and Propolis Associated with Calcium Hydroxide against Single- and Multispecies Biofilms: An In Vitro and In Situ Study
| Autor: | Manoj Reddy Medapati, Rodrigo Sanches Cunha, Rafael Felitti, Christopher Lai, Aldo del Carpio-Perochena, Anjali Y. Bhagirath, Anil Kishen |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science In Vitro Techniques Propolis Enterococcus faecalis Microbiology Calcium Hydroxide Chitosan 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Viability assay Food science General Dentistry Microbial Viability Microscopy Confocal Calcium hydroxide biology Stem Cells Biofilm 030206 dentistry Hydrogen-Ion Concentration biology.organism_classification Anti-Bacterial Agents 030104 developmental biology chemistry Distilled water Biofilms Nanoparticles Dental Pulp Cavity Bacteria |
| Zdroj: | Journal of Endodontics. 43:1332-1336 |
| ISSN: | 0099-2399 |
| DOI: | 10.1016/j.joen.2017.03.017 |
| Popis: | Introduction The aim of this study was to evaluate the efficacy of chitosan nanoparticles (CNPs) and ethanolic propolis extract (EPE) incorporated into a calcium hydroxide paste (Ca[OH] 2 ) to kill bacterial biofilms. Methods Human root canal dentin was infected with Enterococcus faecalis for 21 days and also intraorally for 48 hours followed by incubation in brain-heart infusion for 48 hours to standardize biofilm growth. Ca(OH) 2 pastes associated or not with CNPs or EPE were tested on biofilms for 7 and 14 days. Distilled water was used for control purposes. After the treatment procedures, microbiological analysis was performed to determine the reduction in E. faecalis colonies. Confocal microscopy was used to determine the percentage of cell viability in polymicrobial biofilms before and after the exposure to the experimental intracanal medications. Results All experimental pastes were able to significantly reduce the E. faecalis colony-forming units (CFU) after 7 or 14 days ( P 2 paste ( P 2 showed similar percentages of bacterial viability to the control regardless of the exposure time ( P > .05). The viable cell count significantly dropped in the Ca(OH) 2 /CNPs groups for both 7 and 14 days ( P 2 /EPE groups were only effective in eliminating bacteria during the first 7 days of treatment ( P Conclusions Incorporating CNPs into pastes of Ca(OH) 2 could potentially be beneficial when using interappointment intracanal medications because of their ability to kill bacteria in short- and long-term exposure. |
| Databáze: | OpenAIRE |
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