Taxol Inhibits Opioid Binding on T47D Human Breast Cancer Cells
| Autor: | Elias Castanas, Efsthathia Bakogeorgou, Anastassia Hatzoglou |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Paclitaxel
Ethylketocyclazocine Molecular Sequence Data Biophysics Diprenorphine Breast Neoplasms macromolecular substances Biology Pharmacology Binding Competitive Biochemistry chemistry.chemical_compound Tubulin Tumor Cells Cultured medicine Extracellular Humans Colchicine Amino Acid Sequence Receptor Cytoskeleton Molecular Biology Binding Sites Receptors Opioid kappa Cell Biology Antineoplastic Agents Phytogenic Analgesics Opioid chemistry Receptors Opioid Cancer cell biology.protein Sequence Alignment medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 235:201-204 |
| ISSN: | 0006-291X |
| Popis: | In the T47D human breast cancer cell line, Taxol was found to compete for ethylketocyclazocine opioid binding (IC503.3 pM). In contrast, no interaction of the drug with [3H]diprenorphine binding occurred. Binding was multiphasic, in the absence of colchicine (10−6M), but monophasic in its presence, indicating an involvement of the cytoskeleton in this process. Alignment of Taxol binding domains on alpha and beta tubulin with the kappa opioid site revealed homology of these sites with the first extracellular loop of the receptor. These results indicate a possible new action of Taxol, indicating for the first time a membrane action of the agent. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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