Different Functional Capacities of Latent and Lytic Antigen-Specific CD8 T Cells in Murine Gammaherpesvirus Infection
| Autor: | Sarah G. Crist, Edward J. Usherwood, David C. Gondek, Joshua J. Obar |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Cytotoxicity
Immunologic T cell Immunology Epitopes T-Lymphocyte CD8-Positive T-Lymphocytes Biology Lymphocyte Activation Article Immunophenotyping Mice Interleukin 21 Gammaherpesvirinae Immune system Virus latency medicine Animals Immunology and Allergy Cytotoxic T cell Antigen-presenting cell Antigens Viral Lytic Phase Cells Cultured Interleukin-15 Mice Inbred BALB C Immunodominant Epitopes Interleukin-7 Herpesviridae Infections Cytotoxicity Tests Immunologic medicine.disease Virology Virus Latency medicine.anatomical_structure Lytic cycle Chronic Disease Female |
| Zdroj: | The Journal of Immunology. 172:1213-1219 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.172.2.1213 |
| Popis: | Gammaherpesviruses can persist in the host in the face of an aggressive immune response. T cells recognize Ags expressed in both the productive and latent phases of the virus life cycle, however little is known about their relative roles in the long-term control of the infection. In this study we used the murine gammaherpesvirus 68 model system to investigate the relative properties of CD8 T cells recognizing lytic and latent viral Ags. We report that the CD8 T cell response to lytic phase epitopes is maximal in the lungs of infected mice at ∼10 days postinfection, and is of progressively lesser magnitude in the mediastinal lymph nodes and spleen. In contrast, the CD8 T cell response to the latent M2 protein is maximal at ∼19 days postinfection and is most prominent in the spleen, then progressively less in the mediastinal lymph node and the lung. Latent and lytic Ag-specific CD8 T cells had markedly different cell surface phenotypes during chronic infection, with latent Ag-specific cells being predominantly CD62Lhigh or CD43 (1B11)high. Lytic Ag-specific T cells had significantly lower expression of these markers. Importantly, latent but not lytic Ag-specific T cells could kill target cells rapidly in vivo during the chronic infection. These two different sets of CD8 T cells also responded differentially to IL-7, a cytokine involved in T cell homeostasis and the maintenance of T cell memory. These data have important implications for our understanding of immunological control during chronic gammaherpesvirus infections. |
| Databáze: | OpenAIRE |
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