HIV infection of choroid plexus in AIDS and asymptomatic HIV-infected patients suggests that the choroid plexus may be a reservoir of productive infection
| Autor: | Carol K. Petito, Brenda Roberts, Charles E. Wood, Jorge Torres-Munoz, Hexin Chen, Angeline R Mastri |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
Male HIV Infections Polymerase Chain Reaction Asymptomatic Virus Cellular and Molecular Neuroscience Acquired immunodeficiency syndrome (AIDS) Virology mental disorders medicine Humans Sida Aged Acquired Immunodeficiency Syndrome biology virus diseases Middle Aged medicine.disease biology.organism_classification Immunohistochemistry HIV Envelope Protein gp41 Blotting Southern Neurology Choroid Plexus DNA Viral Immunology Lentivirus HIV-1 Female Choroid plexus Neurology (clinical) Viral disease medicine.symptom Encephalitis |
| Zdroj: | Journal of Neurovirology. 5:670-677 |
| ISSN: | 1538-2443 1355-0284 |
| Popis: | The choroid plexus (CPx) may be an important site of viral dissemination since monocytes and dendritic cells in its stroma are infected with HIV in AIDS patients and since the ratio of CPx to brain infection is more than 2 : 1. In order to see if CPx infection also develops in asymptomatic (ASY) HIV-infected patients, we examined archival formalin-fixed brain and CPx from 14 AIDS and seven ASY cases, using routine histology, immunohistochemistry for HIV gp41, and DNA extraction and gene amplification for HIV DNA. Eight of 14 AIDS (57%) had HIV-positive cells in the CPx and four (29%) had HIV encephalitis. Two of seven ASY cases (29%) had HIV-positive cells in the CPx but none had HIV encephalitis. Extracted DNA from brain, CPx and systemic organs of five ASY cases was amplified by nested PCR with or without Southern blotting for HIV env gene. It was positive in systemic organs in five cases; in CPx in four cases; and in brain in one case. This study shows that the CPx is a site of HIV infection in ASY patients and that the frequency of CPx infection is higher than seen in brain in both AIDS and ASY cases. The results are consistent with the hypothesis that the CPx may be a site for hematogeneous spread and a reservoir for HIV infection during the period of clinical latency. |
| Databáze: | OpenAIRE |
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