Cloning, sequencing and functional expression of zebra (Equus burchelli) LH
| Autor: | F Stewart, M Chopineau, Nadine Martinat, Florian Guillou, C Pourchet, Yves Combarnous |
|---|---|
| Přispěvatelé: | ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 1999 |
| Předmět: |
Male
LH 0106 biological sciences Embryology [SDV]Life Sciences [q-bio] 01 natural sciences Follicle-stimulating hormone Endocrinology FSH HORMONE GONADOTROPE Cloning Molecular ComputingMilieux_MISCELLANEOUS chemistry.chemical_classification 0303 health sciences Leydig cell Reverse Transcriptase Polymerase Chain Reaction EXPRESSION DU GENE Obstetrics and Gynecology Recombinant Proteins Amino acid [SDV] Life Sciences [q-bio] medicine.anatomical_structure COS Cells Biological Assay Gonadotropin Luteinizing hormone hormones hormone substitutes and hormone antagonists endocrine system medicine.medical_specialty medicine.drug_class Molecular Sequence Data [INFO] Computer Science [cs] Molecular cloning Biology 010603 evolutionary biology 03 medical and health sciences Internal medicine medicine Animals [INFO]Computer Science [cs] Amino Acid Sequence Horses 030304 developmental biology Base Sequence BIOCHIMIE Equidae Sequence Analysis DNA Cell Biology Luteinizing Hormone biology.organism_classification Reproductive Medicine chemistry Glycoprotein Hormones alpha Subunit Equus zebra Follicle-stimulating hormone receptor Sequence Alignment |
| Zdroj: | Journal of Reproduction and Fertility Journal of Reproduction and Fertility, Portland Press, 1999, 115, pp.159-166 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/jrf.0.1150159 |
| Popis: | Although donkey luteinizing hormone exhibits a very high degree of amino acid sequence identity with horse LH, its FSH activity in non-equine species is tenfold lower. The coding regions of the common zebra (Equus burchelli) glycoprotein hormone alpha-subunit and LH beta-subunit transcripts were cloned by reverse transcription-PCR from pituitary gland RNA to investigate more precisely the structure-function relationships of this gonadotrophin family. Zebra LH was then expressed in COS-7 cells and its LH and FSH activities were assessed in a rat Leydig cell bioassay (for LH) and in a cell line stably expressing the human FSH receptor bioassay (for FSH). The recombinant zebra LH, although displaying LH activity similar to that of recombinant donkey and horse LH, had no detectable FSH activity. The LH amino acid sequences of these three species are very similar, leaving only very few amino acids as potential candidates to explain the difference in their FSH activities. Moreover, according to the difference in FSH bioactivity and to the percentage identity between the sequences, the common zebra is phylogenetically closer to the donkey than it is to the horse. |
| Databáze: | OpenAIRE |
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