Development and evaluation of rapid point-of-care tests for detection of Enterovirus 71 and Coxsackievirus A16 specific immunoglublin M antibodies
| Autor: | Tong Cheng, Jing Zhang, Hailian Du, Shengxiang Ge, Jun Zhang, Delei He, Ningshao Xia, Shuizhen He, Weng Zuxing, Xu Feihai |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Time Factors Point-of-Care Systems Point-of-care testing 030106 microbiology Antibodies Viral Sensitivity and Specificity Coxsackievirus a16 Chromatography Affinity 03 medical and health sciences Virology Enterovirus 71 Humans Medicine Immunochromatographic Assays Enterovirus Whole blood biology Diagnostic Tests Routine business.industry Serum samples biology.organism_classification Enterovirus A Human Immunoglobulin M Immunology biology.protein Antibody Hand Foot and Mouth Disease business |
| Zdroj: | Journal of Virological Methods. 231:44-47 |
| ISSN: | 0166-0934 |
| Popis: | Two colloidal gold immunochromatographic assays (CGIAs) for detection of EV71- and CA16- immunoglobulin M (IgM) were developed and evaluated. A total of 1465 sera collected from children with hand, foot, and mouth disease (HFMD), non-HFMD patients and healthy children. The sensitivity of IgM CGIA tests for EV71 and CA16 were 97.6% (330/338) and 91.6% (296/323) respectively, compared to those who were viral RNA positive by PCR. Their performances were comparable to those of commercial ELISA kits, with agreement of 98.1% for EV71-IgM and 97.3% for CA16-IgM. In addition, for EV71- and CA16-IgM CGIAs, the results of whole blood samples were 99.6% (248/249) and 100% (191/191) concordant to those with serum samples, respectively. As rapid point-of-care (POC) tests, the two CGIAs were suitable to be used in community clinic units, especially in resource-poor areas and will facilitate the control of HFMD. |
| Databáze: | OpenAIRE |
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