TheKlebsiella pneumoniaeYfgL (BamB) lipoprotein contributes to outer membrane protein biogenesis, type-1 fimbriae expression, anti-phagocytosis, andin vivovirulence
| Autor: | C. H. Chen, Chun-Ru Hsu, Tzu-Lung Lin, Jin-Town Wang, Pei-Fang Hsieh |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Klebsiella pneumoniae Phagocytosis 030106 microbiology Immunology Fimbria Virulence Erythromycin Microbiology 03 medical and health sciences In vivo medicine biology biology.organism_classification Virology Klebsiella Infections 030104 developmental biology Infectious Diseases Parasitology Bacterial outer membrane Biogenesis Research Paper Bacterial Outer Membrane Proteins medicine.drug |
| Zdroj: | Virulence. 7:587-601 |
| ISSN: | 2150-5608 2150-5594 |
| Popis: | Klebsiella pneumoniae is an opportunistic pathogen that causes several kinds of infections, including pneumonia, bacteremia, urinary tract infection and community-acquired pyogenic liver abscess (PLA). Adhesion is the critical first step in the infection process. Our previous work demonstrated that the transcellular translocation is exploited by K. pneumoniae strains to migrate from the gut flora into other tissues, resulting in systemic infections. However, the initial stages of K. pneumoniae infection remain unclear. In this study, we demonstrated that a K. pneumoniae strain deleted for yfgL (bamB) exhibited reduced adherence to and invasion of host cells; changed biogenesis of major β-barrel outer membrane proteins; decreased transcriptional expression of type-1 fimbriae; and increased susceptibility to vancomycin and erythromycin. The yfgL deletion mutant also had reduced ability to against neutrophil phagocytosis; exhibited decreased induction of host IL-6 production; and was profoundly attenuated for virulence in a K. pneumoniae model of bacteremia. Thus, the K. pneumoniae YfgL lipoprotein mediates in outer membrane proteins biogenesis and is crucial for anti-phagocytosis and survival in vivo. These data provide a new insight for K. pneumoniae attachment and such knowledge could facilitate preventive therapies or alternative therapies against K. pneumoniae. |
| Databáze: | OpenAIRE |
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