Fluoride-induced renal dysfunction via respiratory chain complex abnormal expression and fusion elevation in mice

Autor: Bian-hua Zhou, Hong-wei Wang, Jing Liu, Shi-quan Zhu, Yan Zhang, Cheng-yi Miao
Rok vydání: 2019
Předmět:
medicine.medical_specialty
Environmental Engineering
Mitochondrial Diseases
Renal glomerulus
Health
Toxicology and Mutagenesis
0208 environmental biotechnology
Blotting
Western
SDHA
02 engineering and technology
010501 environmental sciences
Kidney
01 natural sciences
Mitochondrial Dynamics
GTP Phosphohydrolases
Electron Transport
Mitochondrial Proteins
Fluorides
Mice
Internal medicine
medicine
Environmental Chemistry
Animals
RNA
Messenger
Renal Insufficiency
0105 earth and related environmental sciences
Cell Proliferation
Chemistry
Respiratory chain complex
Public Health
Environmental and Occupational Health
General Medicine
General Chemistry
medicine.disease
Pollution
020801 environmental engineering
Mitochondria
Disease Models
Animal
medicine.anatomical_structure
Mitochondrial respiratory chain
Endocrinology
mitochondrial fusion
Electron Transport Chain Complex Proteins
Renal papilla
Optic Atrophy 1
Zdroj: Chemosphere. 238
ISSN: 1879-1298
Popis: A fluoride exposure mouse model is established to evaluate the relationship between mitochondrial respiratory chain complexes and renal dysfunction. Morphological changes in kidney tissues were observed. Renal function and cell proliferation in the kidneys were evaluated. The expression of mitochondrial fusion protein including mitofusin-1 (Mfn1) and optic atrophy 1 (OPA1), and mitochondrial respiratory chain complex subunits, including NDUFV2, SDHA, CYC1 and COX Ⅳ, were detected via real-time polymerase chain reaction, immunohistochemistry staining and Western blot, respectively. Results showed that the structures of renal tubule, renal glomerulus and renal papilla were seriously damaged. Renal function was impaired, and cell proliferation was remarkably inhibited by excessive fluoride in kidney. The mRNA and protein expression levels of Mfn1, OPA1, NDUFV2, CYC1 and COX Ⅳ were significantly increased after excessive fluoride exposure. However, the mRNA and protein expression of SDHA significantly decreased. Overall, our findings revealed that excessive fluoride can damage kidney structure, inhibit renal cell proliferation, interfere with the expression of mitochondrial respiratory chain complexes and elevate mitochondrial fusion. Consequently, renal function disorder occurred.
Databáze: OpenAIRE
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