Effects of Combined Treatment with Complex S. typhimurium Antigens and Factors Stimulating Osteogenesis (Curettage, BMP-2) on Multipotent Bone Marrow Stromal Cells and Serum Concentration of Cytokines in CBA Mice
| Autor: | I. M. Shapoval, Anna S. Karyagina, Yu. F. Gorskaya, Vladimir G. Lunin, L. A. Soboleva, M. S. Bartov, A. V. Gromov, V. I. Grabko, T. A. Danilova, V. G. Nesterenko, T. M. Grunina |
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| Rok vydání: | 2015 |
| Předmět: |
Salmonella typhimurium
Stromal cell medicine.medical_treatment Bone Morphogenetic Protein 2 Bone Marrow Cells Bone morphogenetic protein Bone morphogenetic protein 2 General Biochemistry Genetics and Molecular Biology Curettage Andrology Interferon-gamma Mice Immune system Antigen Osteogenesis medicine Animals Cloning Antigens Bacterial Tumor Necrosis Factor-alpha Chemistry General Medicine Interleukin-10 medicine.anatomical_structure Immunology Mice Inbred CBA Cytokines Interleukin-2 Bone marrow Stromal Cells |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 158:465-470 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-015-2786-z |
| Popis: | The content of multipotent stromal cells (MSC) in the bone marrow and efficiency of their cloning (ECF-MSC) increased by 3 times 1 day after administration of complex S. typhimurium antigens to CBA mice, while the relative content of alkaline phosphatase-positive MSC colonies (marker of osteogenesis; P(+) colonies) decreased from 14% (control) to 3%. After administration of the complex S. typhimurium antigens to CBA mice 3 h after (or 3 h before) curettage or treatment with morphogenetic protein (BMP-2), the content of MSC and ECF-MSC decreased on the next day by ~3 times in comparison with animals receiving antigens alone and approached the control level. The relative content of P(+) colonies increased to 20 and 35%, respectively, in comparison with animals receiving antigens (3%), but was significantly lower than after curettage (34%) or BMP-2 (42%) administration. Expression of IL-1β, IL-6, IL-12, TNF-α, and IFN-γ genes in the primary cultures of stromal bone marrow cells induced by antigen administration was suppressed, while the concentrations of IL-12 and TNF-α in the culture medium sharply decreased after antigen treatment in combination with curettage or BMP-2 administration. Administration of complex S. typhimurium antigens after pretreatment with BMP-2 (3 h before) was associated with a decrease in serum levels of IL-2, IFN-γ, IL-12, and TNF-α in mice receiving BMP-2+S. typhimurium group 4 h after treatment in comparison with the animals receiving only S. typhimurium antigens alone by 1.9, 4.4, 1.5, and 6 times, respectively, i.e. to normal level or below it, while the concentration of IL-10 increased by almost 2 times, which probably reflected anti-inflammatory properties of BMP-2. These data probably attest to competitive relations between osteogenesis and immune response at the level of MSC. |
| Databáze: | OpenAIRE |
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