Individuals with HIV-1 subtype C infection and cryptococcal meningitis exhibit viral genetic intermixing of HIV-1 between plasma and cerebrospinal fluid, and a high prevalence of CXCR4-using variants
| Autor: | Richard T. Kangethe, Katlego Sojane, Mahomed Yunus S. Moosa, Thumbi Ndung'u, Sharon R Lewin, Martyn A. French, Christina C. Chang |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Male Receptors CXCR4 Genotype 030106 microbiology Immunology HIV Infections Meningitis Cryptococcal CXCR4 Polymerase Chain Reaction Article law.invention 03 medical and health sciences Plasma Young Adult Cerebrospinal fluid law Virology Genetic variation Blood plasma medicine Prevalence Humans Prospective Studies Cloning Molecular Polymerase chain reaction Tropism Cerebrospinal Fluid business.industry env Gene Products Human Immunodeficiency Virus Genetic Variation Sequence Analysis DNA Middle Aged medicine.disease 030104 developmental biology Infectious Diseases HIV-1 Female business Meningitis |
| Zdroj: | AIDS Research and Human Retroviruses |
| Popis: | The genotypic properties of human immunodeficiency virus type 1 (HIV-1) subtype C in individuals presenting with cryptococcal meningitis (CM) are not well established. Employing single-genome amplification as well as bulk PCR, cloning and sequencing strategies, we evaluated the genetic properties of HIV-1 subtype C env in 16 antiretroviral therapy-naive study participants with CM. Eleven of the 16 participants had matched blood plasma and cerebrospinal fluid (CSF) evaluated, with the rest having either a plasma or CSF sample evaluated. Before antiretroviral therapy initiation, matched plasma and CSF-derived env sequences of all 11 participants displayed genetic intermixing between the two compartments. Overall, 7 of the 16 (∼43.8%) participants harbored CXCR4-using variants in plasma and/or CSF, according to coreceptor usage prediction algorithms. This study suggests that HIV-1 subtype C genetic intermixing between peripheral blood and the central nervous system is common in individuals presenting with CM, and that CXCR4 usage is present in one or both compartments in approximately 44% of individuals. |
| Databáze: | OpenAIRE |
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