Energy budget of Drosophila embryogenesis
| Autor: | Stanislav Y. Shvartsman, Joshua D. Rabinowitz, Yatsuhisa Nagano, Lukas B. Tanner, Yonghyun Song, Junyoung O. Park |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Embryo Nonmammalian 1.1 Normal biological development and functioning Embryonic Development Calorimetry Medical and Health Sciences Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Affordable and Clean Energy Underpinning research Animals Nonmammalian biology Glycogen Hatching Respiration Embryogenesis Psychology and Cognitive Sciences Drosophila embryogenesis Embryo Biological Sciences biology.organism_classification Energy budget Cell biology 030104 developmental biology Drosophila melanogaster chemistry Generic health relevance General Agricultural and Biological Sciences Oviparity Energy Metabolism 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Current biology : CB, vol 29, iss 12 Curr Biol |
| Popis: | Eggs of oviparous animals must be prepared to develop rapidly and robustly until hatching. The balance between sugars, fats, and other macromolecules must therefore be carefully considered when loading the egg with nutrients. Clearly, packing too much or too little fuel would lead to suboptimal conditions for development. While many studies have measured the overall energy utilization of embryos, little is known of the identity of the molecular-level processes that contribute to the energy budget in the first place [1]. Here, we introduce Drosophila embryos as a platform to study the energy budget of embryogenesis. We demonstrate through three orthogonal measurements - respiration, calorimetry, and biochemical assays - that Drosophila melanogaster embryogenesis utilizes 10 mJ of energy generated by the oxidation of the maternal glycogen and triacylglycerol (TAG) stores (Figure 1). Normalized for mass, this is comparable to the resting metabolic rates of insects [2]. Interestingly, alongside data from earlier studies, our results imply that protein, RNA, and DNA polymerization require less than 10% of the total ATPs produced in the early embryo. |
| Databáze: | OpenAIRE |
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