Salmonella-induced Diarrhea Occurs in the Absence of IL-8 Receptor (CXCR2)-Dependent Neutrophilic Inflammation
| Autor: | Ronald R. Marchelletta, Joshua Fierer, Sharon Okamoto, Mélanie G. Gareau, Kim E. Barrett, Donald G. Guiney |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Salmonella Neutrophils colitis medicine.disease_cause Severity of Illness Index Medical and Health Sciences Mice Cecum Receptors 2.1 Biological and endogenous factors Immunology and Allergy Aetiology Inbred BALB C Mice Knockout Mice Inbred BALB C Salmonella enterica neutrophil Interleukin ion transporter Biological Sciences Foodborne Illness Diarrhea Infectious Diseases medicine.anatomical_structure Salmonella Infections Female medicine.symptom Infection Colon Knockout Inflammation Biology Microbiology Vaccine Related Major Articles and Brief Reports Biodefense medicine Animals Interleukin 8 Colitis Receptors Interleukin-8 Animal epithelial cell Prevention Interleukin-8 medicine.disease biology.organism_classification Disease Models Animal Emerging Infectious Diseases Disease Models Immunology Digestive Diseases |
| Zdroj: | The Journal of infectious diseases, vol 212, iss 1 |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | Background. Gastroenteritis is the most common manifestation of nontyphoidal Salmonella enterica infections, but little is known about the pathogenesis of diarrhea in this infection Methods. To determine whether polymorphonuclear neutrophils (PMNs) are required for diarrhea for Salmonella colitis, we infected kanamycin-pretreated interleukin 8R (IL-8R) mutant mice and controls, both with nonmutant Slc11a1 (Nramp1, ItyR). We compared the 2 mouse strains for increases in fecal water content (diarrhea) 3 days after infection, changes in expression of ion transporters in colonic epithelial cells, proliferation of epithelial cells, and severity of infection as measured by colony-forming units (CFUs). Results. The IL-8R knockout mice had fewer PMNs in the colon but the other variables we measured were unaffected except for an increase in CFUs in the colon. The pathologic changes in the cecum were similar in both groups except for the lack of PMNs in the IL-8R knockout mice. There was minimal damage to the colon more distally. Conclusions. In the early stage of Salmonella colitis, PMNs are not required for diarrhea or for the decrease in expression of colonic epithelial cell apical ion transporters. They contribute to defense against infection in the cecum but not extracolonically at this stage of Salmonella colitis. |
| Databáze: | OpenAIRE |
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