Modeling breast cancer by grafting patient tumor samples in the avian embryo: an in vivo platform for therapy evaluation coupled to large scale molecular analyses
| Autor: | Loraine Jarrosson, Clélia Costechareyre, Fanny Gallix, Séverine Ciré, Fabien Gay, Olivier Imbaud, Elisabetta Marangoni, Karine Aguéra, Céline Delloye-Bourgeois, Valérie Castellani |
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| Přispěvatelé: | Institut NeuroMyoGène (INMG), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Therapy Evaluation business.industry [SDV]Life Sciences [q-bio] Cancer Avian embryo medicine.disease 3. Good health law.invention Human tumor 03 medical and health sciences 0302 clinical medicine Breast cancer Confocal microscopy law In vivo 030220 oncology & carcinogenesis Cancer research medicine business Triple-negative breast cancer 030304 developmental biology |
| Popis: | Lack of preclinical patient-derived xenograft (PDX) cancer models in which to conduct large scale molecular studies seriously impairs the development of effective personalized therapies. We report here on an in vivo concept consisting of implanting human tumor cells in targeted tissues of an avian embryo, delivering therapeutics, evaluating their efficacy by measuring tumors using light sheet confocal microscopy, and conducting large scale RNAseq analysis to characterize therapeutic-induced changes in gene expression. The model was established to recapitulate triple negative breast cancer (TNBC) and validated using TNBC standards of care (SOCs) and an investigational therapeutic agent. |
| Databáze: | OpenAIRE |
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