Antibiotic resistance in human peri-implantitis microbiota
| Autor: | Thomas E. Rams, Arie Jan van Winkelhoff, John E. Degener |
|---|---|
| Přispěvatelé: | Microbes in Health and Disease (MHD), Man, Biomaterials and Microbes (MBM), Personalized Healthcare Technology (PHT) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male antibiotic resistance medicine.drug_class Antibiotics submucosal microbiota Drug resistance Microbial Sensitivity Tests Biology SUSCEPTIBILITY PUTATIVE PERIODONTAL PATHOGENS Microbiology Antibiotic resistance Ciprofloxacin Metronidazole PORPHYROMONAS-GINGIVALIS medicine Humans Aged Retrospective Studies Aged 80 and over Clindamycin Amoxicillin Drug Resistance Microbial in vitro IN-VITRO Middle Aged Antimicrobial INFECTIONS Biofilms Doxycycline DISEASES ADULT PERIODONTITIS BONE LOSS DENTAL IMPLANTS Female PARTIALLY EDENTULOUS PATIENTS Oral Surgery medicine.drug peri-implantitis |
| Zdroj: | CLINICAL ORAL IMPLANTS RESEARCH, 25(1), 82-90. Wiley |
| ISSN: | 0905-7161 |
| DOI: | 10.1111/clr.12160 |
| Popis: | ObjectivesBecause antimicrobial therapy is often employed in the treatment of infectious dental implant complications, this study determined the occurrence of in vitro antibiotic resistance among putative peri-implantitis bacterial pathogens.MethodsSubmucosal biofilm specimens were cultured from 160 dental implants with peri-implantitis in 120 adults, with isolated putative pathogens identified to species level, and tested in vitro for susceptibility to 4mg/l of doxycycline, 8mg/l of amoxicillin, 16mg/l of metronidazole, and 4mg/l of clindamycin. Findings for amoxicillin and metronidazole were combined post-hoc to identify peri-implantitis species resistant to both antibiotics. Gram-negative enteric rods/pseudomonads were subjected to ciprofloxacin disk diffusion testing.ResultsOne or more cultivable submucosal bacterial pathogens, most often Prevotella intermedia/nigrescens or Streptococcus constellatus, were resistant in vitro to clindamycin, amoxicillin, doxycycline, or metronidazole in 46.7%, 39.2%, 25%, and 21.7% of the peri-implantitis subjects, respectively. Only 6.7% subjects revealed submucosal test species resistant in vitro to both amoxicillin and metronidazole, which were either S.constellatus (one subject) or ciprofloxacin-susceptible strains of gram-negative enteric rods/pseudomonads (seven subjects). Overall, 71.7% of the 120 peri-implantitis subjects exhibited submucosal bacterial pathogens resistant in vitro to one or more of the tested antibiotics.ConclusionsPeri-implantitis patients frequently yielded submucosal bacterial pathogens resistant in vitro to individual therapeutic concentrations of clindamycin, amoxicillin, doxycycline, or metronidazole, but only rarely to both amoxicillin and metronidazole. Due to the wide variation in observed drug resistance patterns, antibiotic susceptibility testing of cultivable submucosal bacterial pathogens may aid in the selection of antimicrobial therapy for peri-implantitis patients. |
| Databáze: | OpenAIRE |
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