Over-expression of the Bacterial Phytase US417 in Arabidopsis Reduces the Concentration of Phytic Acid and Reveals Its Involvement in the Regulation of Sulfate and Phosphate Homeostasis and Signaling
| Autor: | Stéphane Mari, Pierre Berthomieu, Ameny Farhat, Catherine Curie, Ikram Zaidi, Hichem Chouayekh, Hatem Rouached, Khaled Masmoudi, Jean-Claude Davidian, Nadia Bouain, Sandrine Chay, Nibras Belgaroui, Moez Hanin |
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| Přispěvatelé: | Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Centre de Biotechnologie de Sfax (CBS), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Laboratoire d'Informatique, Signaux, et Systèmes de Sophia-Antipolis (I3S) / Equipe IMAGES-CREATIVE, Signal, Images et Systèmes (Laboratoire I3S - SIS), Laboratoire d'Informatique, Signaux, et Systèmes de Sophia Antipolis (I3S), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Laboratoire d'Informatique, Signaux, et Systèmes de Sophia Antipolis (I3S), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
[SDV.BIO]Life Sciences [q-bio]/Biotechnology Physiology [SDV]Life Sciences [q-bio] Plant Science Plant Roots 01 natural sciences chemistry.chemical_compound Gene Expression Regulation Plant Arabidopsis homeostasis Phosphate Transport Proteins 2. Zero hunger 6-Phytase 0303 health sciences Sulfates food and beverages Phosphorus General Medicine Plants Genetically Modified Sulfate transport Biochemistry Phytic acid Seeds Plant Shoots Signal Transduction Iron chemistry.chemical_element Context (language use) Biology Phosphates 03 medical and health sciences Bacterial Proteins [SDV.BV]Life Sciences [q-bio]/Vegetal Biology acide phosphatique 030304 developmental biology homéostasie Arabidopsis Proteins Cell Biology biology.organism_classification Phosphate Phytase Sulfate Signaling arabidopsis Ion homeostasis chemistry Seedlings 010606 plant biology & botany |
| Zdroj: | Plant and Cell Physiology Plant and Cell Physiology, Oxford University Press (OUP), 2014, 55 (11), pp.1912-24. ⟨10.1093/pcp/pcu122⟩ Plant and Cell Physiology, Oxford University Press (OUP), 2014, 55 (11), pp.1912-1924. ⟨10.1093/pcp/pcu122⟩ |
| ISSN: | 0032-0781 1471-9053 |
| Popis: | International audience; Phytic acid (PA) is the main phosphorus storage form in plant seeds. It is recognized as an anti-nutrient for humans and non-ruminant animals, as well as one of the major sources of phosphorus that contributes to eutrophication. Therefore, engineering plants with low PA content without affecting plant growth capacity has become a major focus in plant breeding. Nevertheless, lack of knowledge on the role of PA seed reserves in regulating plant growth and in maintaining ion homeostasis hinders such an agronomical application. In this context, we report here that the over-expression of the bacterial phytase PHY-US417 in Arabidopsis leads to a significant decrease in seed PA, without any effect on the seed germination potential. Interestingly, this over-expression also induced a higher remo-bilization of free iron during germination. Moreover, the PHY-over-expressor lines show an increase in inorganic phosphate and sulfate contents, and a higher biomass production after phosphate starvation. Finally, phosphate sensing was altered because of the changes in the expression of genes induced by phosphate starvation or involved in phosphate or sulfate transport. Together, these results show that the over-expression of PHY-US417 reduces PA concentration, and provide the first evidence for the involvement of PA in the regulation of sulfate and phosphate homeostasis and signaling. |
| Databáze: | OpenAIRE |
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