Expression of FKBP52 in the ovaries of PCOS rats
| Autor: | Yong Tan, Shiyan Song |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway MAP Kinase Signaling System H&E stain Gene Expression Estrous Cycle Ovary Tacrolimus Binding Proteins Andrology 03 medical and health sciences 0302 clinical medicine Genes Reporter androgen receptor Genetics medicine Animals Vaginal smear Gene silencing Gene Silencing RNA Messenger RNA Small Interfering polycystic ovarian syndrome Oncogene Chemistry Kinase Estrogens FK-506 binding protein 52 Articles General Medicine Immunohistochemistry Rats Disease Models Animal 030104 developmental biology medicine.anatomical_structure Receptors Androgen 030220 oncology & carcinogenesis Female adenovirus vectors mitogen-activated protein kinase/extracellular signal-regulated kinase pathway Polycystic Ovary Syndrome Signal Transduction |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X 1107-3756 |
| DOI: | 10.3892/ijmm.2018.3998 |
| Popis: | The present study aimed to examine the expression of FK-506 binding protein 52 (FKBP52) in the ovary tissues of rats with polycystic ovarian syndrome (PCOS) and its action on mediating androgen receptor (AR) through the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathway. PCOS model rats were established by dehydroepiandrosterone injection. Enzyme-linked immunosorbent assay (ELISA) measured serum sex hormones. Hematoxylin and eosin (H&E) staining was used to examine histological changes of the ovarian tissues. The expression levels of FKBP52 were detected by immunohistochemical (IHC) staining, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis and western blotting (WB). In addition, RT-qPCR analysis was used to detect the mRNA expression of AR, and WB was used to detect the protein expression levels of AR, ERK1/2 and phosphorylated (p-) ERK1/2. In granulosa cell (GC) experiments, primary GCs were extracted and cultured. FKBP4 is the FKBP52-encoding gene, therefore, adenovirus vectors Ad-Oe-FKBP4-EGFP and Ad-siRNA-FKBP4-EGFP were constructed to examine the association among the above factors using the RT-qPCR and WB methods. In the animal experiment, the vaginal smear, H&E staining and ELISA results showed that the PCOS model was successfully established. The IHC staining revealed that the expression of FKBP52 in the GCs of the PCOS model group was higher than the remaining groups (P |
| Databáze: | OpenAIRE |
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