Safety of intradiscal injection and biocompatibility of polyester amide microspheres in a canine model predisposed to intervertebral disc degeneration

Autor: Detlef Schumann, George Mihov, Maarten van Dijk, Clemens Bos, Guy C. M. Grinwis, Laura B. Creemers, Marianna A. Tryfonidou, Nicole Willems, Wouter J.A. Dhert, Björn P. Meij, Gustav J. Strijkers
Přispěvatelé: ACS - Atherosclerosis & ischemic syndromes, Biomedical Engineering and Physics, ACS - Heart failure & arrhythmias
Rok vydání: 2015
Předmět:
musculoskeletal diseases
medicine.medical_specialty
Pathology
Materials science
Biocompatibility
Polyesters
intradiscal delivery
Cell
Drug Evaluation
Preclinical
Biomedical Engineering
Apoptosis
Intervertebral Disc Degeneration
02 engineering and technology
Degeneration (medical)
Original Research Report
nucleus pulpo-sus
Biomaterials
Mice
03 medical and health sciences
Dogs
0302 clinical medicine
Original Research Reports
Materials Testing
Journal Article
medicine
Animals
Cytotoxicity
bcl-2-Associated X Protein
030203 arthritis & rheumatology
nucleus pulposus
biomaterial
Intervertebral disc
musculoskeletal system
021001 nanoscience & nanotechnology
Disease Models
Animal
microspheres
medicine.anatomical_structure
Gene Expression Regulation
dog
Immunohistochemistry
Histopathology
Rabbits
0210 nano-technology
Biomedical engineering
Zdroj: Journal of Biomedical Materials Research. Part B, Applied Biomaterials., 105(4), 707. John Wiley and Sons Inc.
Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Journal of biomedical materials research. Part B, Applied biomaterials, 105(4), 707-714. John Wiley and Sons Inc.
ISSN: 1552-4981
1552-4973
Popis: Repair of degenerated intervertebral discs (IVD) might be established via intradiscal delivery of biologic therapies. Polyester amide polymers (PEA) were evaluated for in vitro cytotoxicity and in vivo biocompatibility, and thereafter intradiscal application of PEA microspheres (PEAMs) in a canine model predisposed to IVD degeneration at long‐term (6 months) follow‐up. PEA extracts did not induce cytotoxicity in mouse fibroblast cells (microscopy and XTT assay), while a slight foreign body reaction was demonstrated by histopathology after intramuscular implantation in rabbits. Intradiscal injection of a volume of 40 µL through 26 and 27G needles induced no degenerative changes in acanine model susceptible to IVD disease. Although sham‐injected IVDs showed increased CAV1 expression compared with noninjected IVDs, which may indicate increased cell senescence, these findings were not supported by immunohistochemistry, biomolecular analysis of genes related to apoptosis, biochemical and histopathological results. PEAM‐injected IVDs showed significantly higher BAX/BCL2 ratio vs sham‐injected IVDs suggestive of an anti‐apoptotic effect of the PEAMs. These findings were not supported by other analyses (clinical signs, disc height index, T2 values, biomolecular and biochemical analyses, and IVD histopathology). PEAs showed a good cytocompatibility and biocompatibility. PEAMs are considered safe sustained release systems for intradiscal delivery of biological treatments. © 2015 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 707–714, 2017.
Databáze: OpenAIRE
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