Poly(trimethylene carbonate-co-L-lactide) electrospun scaffolds for use as vascular grafts

Autor: M L Dias, Daikelly Iglesias Braghirolli, Jéssica F. T. C. Petry, Patricia Pranke, Douglas Gamba, B Caberlon
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Scaffold
Physiology
Polyesters
Immunology
Myocytes
Smooth Muscle
Biophysics
Ocean Engineering
Context (language use)
Biochemistry
Dioxanes
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Tissue engineering
Materials Testing
Humans
General Pharmacology
Toxicology and Pharmaceutics
lcsh:QH301-705.5
Cells
Cultured
Endothelial progenitor cells
Cell Proliferation
lcsh:R5-920
Lactide
Tissue Scaffolds
General Neuroscience
Poly(trimethylene carbonate-co-L-lactide)
Mesenchymal stem cell
Mesenchymal Stem Cells
Cell Biology
General Medicine
Vascular scaffolds
Electrospinning
Blood Vessel Prosthesis
Polyester
030104 developmental biology
lcsh:Biology (General)
chemistry
Smooth muscle cells
030220 oncology & carcinogenesis
Mesenchymal stem cells
Trimethylene carbonate
lcsh:Medicine (General)
Biomedical engineering
Research Article
Zdroj: Brazilian Journal of Medical and Biological Research, Volume: 52, Issue: 8, Article number: e8318, Published: 12 AUG 2019
Brazilian Journal of Medical and Biological Research
Brazilian Journal of Medical and Biological Research v.52 n.8 2019
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Brazilian Journal of Medical and Biological Research, Vol 52, Iss 8
Popis: Currently, there is great clinical need for suitable synthetic grafts that can be used in vascular diseases. Synthetic grafts have been successfully used in medium and large arteries, however, their use in small diameter vessels is limited and presents a high failure rate. In this context, the aim of this study was to develop tissue engineering scaffolds, using poly(trimethylene carbonate-co-L-lactide) (PTMCLLA), for application as small diameter vascular grafts. For this, copolymers with varying trimethylene carbonate/lactide ratios – 20/80, 30/70, and 40/60 – were submitted to electrospinning and the resulting scaffolds were evaluated in terms of their physicochemical and biological properties. The scaffolds produced with PTMCLLA 20/80, 30/70, and 40/60 showed smooth fibers with an average diameter of 771±273, 606±242, and 697±232 nm, respectively. When the degradation ratio was evaluated, the three scaffold groups had a similar molecular weight (M w) on the final day of analysis. PTMCLLA 30/70 and 40/60 scaffolds exhibited greater flexibility than the PTMCLLA 20/80. However, the PTMCLLA 40/60 scaffolds showed a large wrinkling and their biological properties were not evaluated. The PTMCLLA 30/70 scaffolds supported the adhesion and growth of mesenchymal stem cells (MSCs), endothelial progenitor cells, and smooth muscle cells (SMCs). In addition, they provided a spreading of MSCs and SMCs. Given the results, the electrospun scaffolds produced with PTMCLLA 30/70 copolymer can be considered promising candidates for future applications in vascular tissue engineering.
Databáze: OpenAIRE
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