Photophysics and ex vivo biodistribution of β-cyclodextrin-meso-tetra(m-hydroxyphenyl)porphyrin conjugate for biomedical applications
| Autor: | Vladimir Kirejev, A. R. Goncalves, Jerker Mårtensson, I. Manet, Konstantina Yannakopoulou, Marica B. Ericson, C. Aggelidou |
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| Rok vydání: | 2014 |
| Předmět: |
Biodistribution
Fluorescence-lifetime imaging microscopy Porphyrins medicine.medical_treatment Biological Transport Active Photodynamic therapy In Vitro Techniques Photochemistry PDR chemistry.chemical_compound Cell Line Tumor medicine Fluorescence microscope Humans Photosensitizer Physical and Theoretical Chemistry Skin cyclodextrins Photosensitizing Agents Chemistry beta-Cyclodextrins Water Photochemical Processes Porphyrin Squamous carcinoma Microscopy Fluorescence Multiphoton Spectrometry Fluorescence Photochemotherapy Solubility Spectrophotometry Carcinoma Squamous Cell Biophysics Female Ex vivo |
| Zdroj: | Photochemical & photobiological sciences 13 (2014): 1185–1191. doi:10.1039/c4pp00088a info:cnr-pdr/source/autori:Kirejev, Vladimir; Gonçalves, Alexandrino Ricardo S; Aggelidou, Chrysie; Manet, Ilse; Mårtensson, Jerker; Yannakopoulou, Konstantina; Ericson, Marica B./titolo:Photophysics and ex vivo biodistribution of b-cyclodextrin-meso-tetra(m-hydroxyphenyl)porphyrin conjugate for biomedical applications/doi:10.1039%2Fc4pp00088a/rivista:Photochemical & photobiological sciences (Print)/anno:2014/pagina_da:1185/pagina_a:1191/intervallo_pagine:1185–1191/volume:13 |
| ISSN: | 1474-9092 1474-905X |
| Popis: | Low aqueous solubility of porphyrin-based photosensitizers hampers their clinical use in photodynamic therapy because of complex delivery. In this study, we explore meso-tetra(m-hydroxyphenyl)-21,23H-porphyrin (mTHPP), a potent photosensitizer, covalently attached to ?-cyclodextrin (CD-mTHPP) with a focus on topical delivery and cellular uptake. The photophysical properties of CD-mTHPP were examined using steady-state fluorescence and lifetime measurements verifying increased aqueous solubility. Confocal and fluorescence lifetime imaging microscopy on human squamous carcinoma cells (A431) evidenced a cytoplasmic uptake of CD-mTHPP in predominantly monomeric form. CD-mTHPP was also delivered to human skin ex vivo and the skin penetration was assessed using two-photon fluorescence microscopy. The results indicated that CD-mTHPP exhibits improved skin distribution compared to mTHPP alone using aqueous vehicles. Thus the CD-mTHPP conjugate demonstrates improved biodistribution ex vivo compared to mTHPP and is a promising multimodal system for photodynamic therapy. This journal is © the Partner Organisations 2014. |
| Databáze: | OpenAIRE |
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