Endophytic actinobacteria: Diversity, secondary metabolism and mechanisms to unsilence biosynthetic gene clusters
| Autor: | Raghavan, Dinesh, Veeraraghavan, Srinivasan, Sheeja, T E, Muthuswamy, Anandaraj, Hamza, Srambikkal |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030106 microbiology Secondary Metabolism Computational biology Biology Secondary metabolite Applied Microbiology and Biotechnology Microbiology DNA sequencing Actinobacteria 03 medical and health sciences Metabolic potential Drug Discovery Endophytes medicine Host plants Secondary metabolism Gene Biological Products High-Throughput Nucleotide Sequencing General Medicine Plants biology.organism_classification Mode of entry Multigene Family medicine.drug |
| Zdroj: | Critical Reviews in Microbiology. 43:546-566 |
| ISSN: | 1549-7828 1040-841X |
| DOI: | 10.1080/1040841x.2016.1270895 |
| Popis: | Endophytic actinobacteria, which reside in the inner tissues of host plants, are gaining serious attention due to their capacity to produce a plethora of secondary metabolites (e.g. antibiotics) possessing a wide variety of biological activity with diverse functions. This review encompasses the recent reports on endophytic actinobacterial species diversity, in planta habitats and mechanisms underlying their mode of entry into plants. Besides, their metabolic potential, novel bioactive compounds they produce and mechanisms to unravel their hidden metabolic repertoire by activation of cryptic or silent biosynthetic gene clusters (BGCs) for eliciting novel secondary metabolite production are discussed. The study also reviews the classical conservative techniques (chemical/biological/physical elicitation, co-culturing) as well as modern microbiology tools (e.g. next generation sequencing) that are being gainfully employed to uncover the vast hidden scaffolds for novel secondary metabolites produced by these endophytes, which would subsequently herald a revolution in drug engineering. The potential role of these endophytes in the agro-environment as promising biological candidates for inhibition of phytopathogens and the way forward to thoroughly exploit this unique microbial community by inducing expression of cryptic BGCs for encoding unseen products with novel therapeutic properties are also discussed. |
| Databáze: | OpenAIRE |
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