The Effect of EZH2 Inhibition through DZNep on Epithelial–Mesenchymal Transition Mechanism
Autor: | Hilal Akalin, Eser Kilic, Mevlut Baskol, Merve Özel, Gulden Baskol |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
030102 biochemistry & molecular biology biology Cell growth EZH2 Vimentin Cell Biology 03 medical and health sciences 030104 developmental biology Apoptosis Histone methyltransferase Gene expression biology.protein Cancer research Viability assay Epithelial–mesenchymal transition Developmental Biology Biotechnology |
Zdroj: | Cellular Reprogramming. 23:139-148 |
ISSN: | 2152-4998 2152-4971 |
Popis: | Although the molecular pathogenesis of hepatocellular carcinoma (HCC) is uncertain, it is known that the epithelial-mesenchymal transition (EMT) mechanism and epigenetic changes have an important role. This study was focused on evaluating the relationship of 3-Deazaneplanocin A (DZNep) with the EMT mechanism, which is a histone methyltransferase inhibitor on HCC and is also known as an enhancer of zeste homolog 2 (EZH2) inhibitor. Cell viability of HepG2 cells (HCC cell line) assessed for DZNep over 72 hours with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Additionally, colony-forming assay, apoptosis assay, RNA isolation, cDNA synthesis, and real-time PCR (RT-PCR) were performed to see the effect of DZNep on HepG2 cells. DZNep reduced cell proliferation for 72 hours, also significantly reduced colony formation in addition it increased the total apoptosis. DZNep on EZH2, E-cadherin, N-cadherin, and Vimentin (Vim) gene expressions was given different results by either decreasing or increasing the expressions. In this study, we observed a positive effect of DZNep on apoptosis and TIMP3 expression level and decreased colony formation. However, it gave complicated results with the level of gene expression E-cadherin and TIMP2, increase the level of Vim and MMP2 expression. Therefore, we think that further studies are necessary to clarify the role of DZNep. |
Databáze: | OpenAIRE |
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