Distribution of capsular types and production of muramidase-released protein (MRP) and extracellular factor (EF) of Streptococcus suis strains isolated from diseased pigs in seven European countries
| Autor: | Henk J. Wisselink, Norbert Stockhofe-Zurwieden, U. Vecht, Hilde E. Smith, Klaas Peperkamp |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Serotype
Streptococcus suis Swine Serotypes Blotting Western Colony Count Microbial Virulence Microbiology Serous Membrane Bacterial Proteins Pig-bacteria Agglutination Tests Streptococcal Infections Animals Serotyping Lung Instituut voor Dierhouderij en Diergezondheid Bacterial Capsules Swine Diseases Antiserum Streptococcus suis serotype 2 ID-Lelystad General Veterinary biology Age Factors Antibodies Monoclonal Brain Heart General Medicine biology.organism_classification Streptococcaceae Virology Europe ID Lelystad ID-Lelystad Instituut voor Dierhouderij en Diergezondheid ID Lelystad Institute for Animal Science and Health biology.protein Electrophoresis Polyacrylamide Gel Antibody Institute for Animal Science and Health Bacteria |
| Zdroj: | Veterinary Microbiology, 74, 237-248 Veterinary Microbiology 74 (2000) |
| ISSN: | 0378-1135 |
| Popis: | Streptococcus suis strains (n=411), isolated from diseased pigs in seven European countries were serotyped using specific antisera against serotype 1 to 28, and were phenotyped on the basis of their muramidase-released-protein (MRP) and extracellular-factor protein (EF) production. Overall, S. suis serotype 2 appeared to be most prevalent (32%), followed by serotype 9 (20%) and serotype 1 (12%). Serotype 2 was most frequently isolated in France, Italy and Spain, whereas serotype 9 was most frequently isolated in Belgium, The Netherlands and Germany. In the United Kingdom serotypes 1 and 14 were most frequently isolated. High percentages of S. suis serotype 1, 2, 1/2 and 14 strains, isolated from tissues associated with S. suis infections such as brain, serosa, joint, heart and organs expressed the EF-protein, indicating that in these serotypes expression of EF is likely to be associated with virulence. In contrast, strains belonging to serotype 7 and 9, isolated from tissues associated with S. suis infections did not produce EF. These results strongly suggest that in the serotypes 7 and 9 EF expression is not related to virulence. More than 80% of the S. suis serotype 9 strains produced an MRP* protein, a high molecular variant of the 136kDa MRP. Expression of MRP* in serotype 9 strains is possibly associated with virulence. |
| Databáze: | OpenAIRE |
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