Evaluation of a pan-serotype point-of-care rapid diagnostic assay for accurate detection of acute dengue infection
| Autor: | Murali Krishna Kaja, Anmol Chandele, Ira Khanna, Kaustuv Nayak, Navin Khanna, Syed Fazil Ahamed, Rosario Vivek, Mary Dias, Shalini Kotabagi, Anita Shet |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Serotype Male Adolescent Secondary infection Point-of-Care Systems 030231 tropical medicine 030106 microbiology India Enzyme-Linked Immunosorbent Assay Viral Nonstructural Proteins Antibodies Viral Real-Time Polymerase Chain Reaction Asymptomatic Communicable Diseases Sensitivity and Specificity law.invention Dengue fever Dengue 03 medical and health sciences 0302 clinical medicine law parasitic diseases medicine Humans Prospective Studies Child Antigens Viral Polymerase chain reaction Point of care Rapid diagnostic test business.industry Diagnostic Tests Routine Infant General Medicine Dengue Virus medicine.disease Virology Infectious Diseases Immunoglobulin M Child Preschool Cohort Female medicine.symptom business |
| Zdroj: | Diagnostic microbiology and infectious disease. 87(3) |
| ISSN: | 1879-0070 |
| Popis: | The catastrophic rise in dengue infections in India and globally has created a need for an accurate, validated low-cost rapid diagnostic test (RDT) for dengue. We prospectively evaluated the diagnostic performance of NS1/IgM RDT (dengue day 1) using 211 samples from a pediatric dengue cohort representing all 4 serotypes in southern India. The dengue-positive panel consisted of 179 dengue real-time polymerase chain reaction (RT-PCR) positive samples from symptomatic children. The dengue-negative panel consisted of 32 samples from dengue-negative febrile children and asymptomatic individuals that were negative for dengue RT-PCR/NS1 enzyme-linked immunosorbent assay/IgM/IgG. NS1/IgM RDT sensitivity was 89.4% and specificity was 93.8%. The NS1/IgM RDT showed high sensitivity throughout the acute phase of illness, in primary and secondary infections, in different severity groups, and detected all 4 dengue serotypes, including coinfections. This NS1/IgM RDT is a useful point-of-care assay for rapid and reliable diagnosis of acute dengue and an excellent surveillance tool in our battle against dengue. |
| Databáze: | OpenAIRE |
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