Direct low field J-edited diffusional proton NMR spectroscopic measurement of COVID-19 inflammatory biomarkers in human serum
Autor: | Philipp Nitschke, Samantha Lodge, Drew Hall, Hartmut Schaefer, Manfred Spraul, Nieves Embade, Oscar Millet, Elaine Holmes, Julien Wist, Jeremy K. Nicholson |
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Rok vydání: | 2022 |
Předmět: |
BLOOD
Biochemistry Analytical Chemistry INFECTION 0399 Other Chemical Sciences Electrochemistry URINE SPECTRA Humans Environmental Chemistry NUCLEAR-MAGNETIC-RESONANCE Phospholipids Spectroscopy Science & Technology IDENTIFICATION SARS-CoV-2 Chemistry Analytical COVID-19 PLASMA-LIPOPROTEINS GLYCOPROTEINS Chemistry GLYCA Physical Sciences H-1-NMR SPECTROSCOPY Protons 0301 Analytical Chemistry Biomarkers |
Zdroj: | The Analyst. 147:4213-4221 |
ISSN: | 1364-5528 0003-2654 |
Popis: | A JEDI NMR pulse experiment incorporating relaxational, diffusional and J-modulation peak editing has been implemented for a low field (80 MHz proton resonance frequency) spectrometer system to measure quantitatively two recently discovered plasma markers of SARS-CoV-2 infection and general inflammation. JEDI spectra capture a unique signature of two biomarker signals from acetylated glycoproteins (Glyc) and the supramolecular phospholipid composite (SPC) signals that are relatively enhanced by the combination of relaxation, diffusion and J-editing properties of the JEDI experiment that strongly attenuate contributions from the other molecular species in plasma. The SPC/Glyc ratio data were essentially identical in the 600 MHz and 80 MHz spectra obtained (R2 = 0.97) and showed significantly different ratios for control (n = 28) versus SARS-CoV-2 positive patients (n = 29) (p = 5.2 × 10-8 and 3.7 × 10-8 respectively). Simplification of the sample preparation allows for data acquisition in a similar time frame to high field machines (∼4 min) and a high-throughput version with 1 min experiment time could be feasible. These data show that these newly discovered inflammatory biomarkers can be measured effectively on low field NMR instruments that do not not require housing in a complex laboratory environment, thus lowering the barrier to clinical translation of this diagnostic technology. |
Databáze: | OpenAIRE |
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