Large-scale purification of IgM from human sera. Comparison of three optimized procedures utilizing protein A chromatography
| Autor: | Hans-Joachim Hammer, Gunther Kümel, Harald Mauch |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Immunodiffusion
Chromatography biology Chemistry General Medicine Boric acid chemistry.chemical_compound Zinc Boric Acids Immunoglobulin M Yield (chemistry) biology.protein Chromatography Gel Chemical Precipitation Humans Electrophoresis Polyacrylamide Gel Waldenstrom Macroglobulinemia Protein A Staphylococcal Protein A Ultracentrifugation Gram |
| Zdroj: | Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie. 177(1) |
| ISSN: | 0300-9130 |
| Popis: | for the preparation of gram amounts of IgM from human sera sedimentation at 100,000 g or treatment with ZnSO4 of the redissolved "euglobulin"-precipitate was compared to direct precipitation from the clarified serum by boric acid. Three alternative large scale purification procedures were developed, leading to an IgM-sample characterized as pure by various criteria. Inclusion of protein A chromatography proved to enhance the yield very considerably. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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