Proteasome subunit RPT2a promotes PTGS through repressing RNA quality control in Arabidopsis
| Autor: | Seulbee Lee, Jeong Mee Park, Myung-Hee Kim, Jieun Jeon, Byung-Hoon Lee, Yun Ju Kim, June M. Kwak, Jae Ho Lee, Lei Gao |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Proteasome Endopeptidase Complex Protein subunit Transgene Arabidopsis Endogeny Plant Science 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Gene silencing Transgenes biology Arabidopsis Proteins fungi RNA biology.organism_classification Cell biology 030104 developmental biology Proteasome RNA Plant RNA Interference Biogenesis 010606 plant biology & botany |
| Zdroj: | Nature Plants. 5:1273-1282 |
| ISSN: | 2055-0278 |
| Popis: | RNA quality control (RQC) and post-transcriptional gene silencing (PTGS) target and degrade aberrant endogenous RNAs and foreign RNAs, contributing to homeostasis of cellular RNAs. In plants, RQC and PTGS compete for foreign and selected endogenous RNAs; however, little is known about the mechanism interconnecting the two pathways. Using a reporter system designed for monitoring PTGS, we revealed that the 26S proteasome subunit RPT2a enhances transgene PTGS by promoting the accumulation of transgene-derived short interfering RNAs without affecting their biogenesis. RPT2a physically associated with a subset of RQC components and downregulated the protein level. Overexpression of the RQC components interfered with transgene silencing, and impairment of the RQC machinery reinforced transgene PTGS attenuated by rpt2a. Overall, we demonstrate that the 26S proteasome subunit RPT2a promotes PTGS by repressing the RQC machinery to control foreign RNAs. The 26S proteasome subunit RPT2a is reported to promote post-transcriptional gene silencing by repressing the RNA quality control machinery, and thereby interconnecting the two pathways that regulate cellular RNA homeostasis in plants. |
| Databáze: | OpenAIRE |
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