Sugar transport for enhanced xylose utilization in Ashbya gossypii
| Autor: | Victoria Isabel Martín, Alberto Jiménez, David Díaz-Fernández, José L. Revuelta, Gloria Muñoz-Fernández |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Monosaccharide Transport Proteins
Pentoses Pentose Bioengineering Fungus Xylose Sugar transport Applied Microbiology and Biotechnology chemistry.chemical_compound Genetics and Molecular Biology of Industrial Organisms - Short Communication Ashbya gossypii Hexose Clustered Regularly Interspaced Short Palindromic Repeats Amino acid replacement Gene CRISPR/Cas9 chemistry.chemical_classification biology Chemistry biology.organism_classification Yeast Glucose Biochemistry Fermentation Biotechnology |
| Zdroj: | Journal of Industrial Microbiology & Biotechnology GREDOS. Repositorio Institucional de la Universidad de Salamanca instname |
| ISSN: | 1476-5535 1367-5435 |
| Popis: | The co-utilization of mixed (pentose/hexose) sugars constitutes a challenge for microbial fermentations. The fungus Ashbya gossypii, which is currently exploited for the industrial production of riboflavin, has been presented as an efficient biocatalyst for the production of biolipids using xylose-rich substrates. However, the utilization of xylose in A. gossypii is hindered by hexose sugars. Three A. gossypii homologs (AFL204C, AFL205C and AFL207C) of the yeast HXT genes that code for hexose transporters have been identified and characterized by gene-targeting approaches. Significant differences in the expression profile of the HXT homologs were found in response to different concentrations of sugars. More importantly, an amino acid replacement (N355V) in AFL205Cp, introduced by CRISPR/Cas9-mediated genomic edition, notably enhanced the utilization of xylose in the presence of glucose. Hence, the introduction of the afl205c-N355V allele in engineered strains of A. gossypii will further benefit the utilization of mixed sugars in this fungus. Electronic supplementary material The online version of this article (10.1007/s10295-020-02320-5) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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