Fructose Consumption Affects Glucocorticoid Signaling in the Liver of Young Female Rats
| Autor: | Mihajlo B. Spasić, Aleksandra Nikolić-Kokić, Jelena Brkljačić, Alhadi M Glban, Ana Djordjevic, Sanja Kovačević, Ivana Elaković, Gordana Matić, Danijela Vojnović Milutinović, Luc Tappy |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty lipin-1 030209 endocrinology & metabolism lcsh:TX341-641 Fructose Fructokinase Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Glucocorticoid receptor Receptors Glucocorticoid antioxidant enzymes Internal medicine 11-beta-Hydroxysteroid Dehydrogenase Type 1 medicine Dietary Carbohydrates glucocorticoid receptor Animals Rats Wistar Glucocorticoids Triglycerides 2. Zero hunger Nutrition and Dietetics biology Chemistry Aldolase B Lipogenesis Nuclear Proteins Rats 030104 developmental biology Endocrinology Gluconeogenesis Liver inflammation biology.protein GLUT2 Female fructose-fed rat Phosphoenolpyruvate carboxykinase Corticosterone lcsh:Nutrition. Foods and food supply Glucocorticoid Food Science medicine.drug Signal Transduction |
| Zdroj: | Nutrients Nutrients, Vol 12, Iss 3470, p 3470 (2020) Volume 12 Issue 11 |
| ISSN: | 2072-6643 |
| Popis: | The effects of early-life fructose consumption on hepatic signaling pathways and their relation to the development of metabolic disorders in later life are not fully understood. To investigate whether fructose overconsumption at a young age induces alterations in glucocorticoid signaling that might contribute to development of metabolic disturbances, we analysed glucocorticoid receptor hormone-binding parameters and expression of its target genes involved in gluconeogenesis (phosphoenolpyruvate carboxykinase and glucose-6-phosphatase) and lipid metabolism (lipin-1), as well as redox and inflammatory status in the liver of female rats subjected to a fructose-rich diet immediately after weaning. The fructose diet increased hepatic corticosterone concentration, 11&beta hydroxysteroid dehydrogenase type 1 level, glucocorticoid receptor protein level and hormone-binding activity, as well as lipin-1 level. The expression of glucose-6-phosphatase was reduced in fructose-fed rats, while phosphoenolpyruvate carboxykinase remained unaltered. The fructose-rich diet increased the level of fructose transporter GLUT2, while the expression of fructolytic enzymes fructokinase and aldolase B remained unaltered. The diet also affected pro-inflammatory pathways, but had no effect on the antioxidant defence system. In conclusion, a fructose-rich diet applied immediately after weaning promoted lipogenesis and enhanced hepatic glucocorticoid signaling, possibly to protect against inflammatory damage, but without an effect on gluconeogenesis and antioxidant enzymes. Yet, prolonged treatment might ultimately lead to more pronounced metabolic disturbances. |
| Databáze: | OpenAIRE |
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