Discrimination between fiber populations in mammalian skeletal muscle by using ultrastructural parameters
| Autor: | Aileen M. Kuda, Brenda R. Eisenberg |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Population
Guinea Pigs Statistics as Topic Biology medicine Animals Fiber Terminal cisternae education Molecular Biology Soleus muscle education.field_of_study musculoskeletal neural and ocular physiology Endoplasmic reticulum Muscles Skeletal muscle Anatomy musculoskeletal system Line width Mitochondria body regions Sarcoplasmic Reticulum medicine.anatomical_structure Ultrastructure tissues |
| Zdroj: | Journal of ultrastructure research. 54(1) |
| ISSN: | 0022-5320 |
| Popis: | Stereological techniques are used to obtain quantitative information from electron micrographs from 300 fibers of skeletal muscle from the adult guinea pig. Ultrastructural parameters measured include Z line width, volume of mitochondria, volume and surface area of tsystem, terminal cisternae, and sarcoplasmic reticulum. Histograms, scattergrams, and statistical discriminant analysis are used to analyze the data. The whole fiber population can be separated with 90% success rate into the red and white vastus muscles and the soleus muscle, which are physiologically fast and slow, respectively. The soleus fibers have a Z line wider than 1100 A and smaller amounts of t-system and terminal cisternae than the vastus fibers. The mitochondrial volume of the vastus fibers shows a continuous distribution over a large range (0.1–15%), with the white vastus fibers at the lower end and the red vastus fibers at the higher end. |
| Databáze: | OpenAIRE |
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