Molecular Analysis ofHuman MetapneumovirusDetected in Patients with Lower Respiratory Tract Infection in Upper Egypt
| Autor: | Mohamed S. Badary, Brunhilde Schweiger, Wolfram Brune, Janine Reiche, Ahmed H. Osmann, Amany G. Thabit, Mona Embarek Mohamed, Sonja Jacobsen |
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| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
education.field_of_study Pathology medicine.medical_specialty Lineage (genetic) Article Subject biology Respiratory tract infections Sequence analysis viruses Population virus diseases biology.organism_classification medicine.disease Microbiology Virology QR1-502 Virus respiratory tract diseases Human metapneumovirus Lower respiratory tract infection medicine education Pathogen Research Article |
| Zdroj: | International Journal of Microbiology International Journal of Microbiology, Vol 2014 (2014) |
| ISSN: | 1687-9198 1687-918X |
| Popis: | Introduction. Since 2001, whenHuman metapneumovirus(HMPV) was isolated in the Netherlands, the virus has been detected in several continents. Although reports have confirmed the prevalence of HMPV worldwide, data from Egypt remain limited. HMPV plays an important role in respiratory tract infections in individuals of all ages particularly in children. This study was aimed at estimating the prevalence of HMPV in patients with community-acquired lower respiratory infection in Upper Egypt and characterizing the circulating Egyptian HMPV strains for the first time.Materials and Methods. From 2005 to 2008, respiratory samples from 520 patients were analyzed for the presence of HMPV by real-time RT-PCR. Molecular and phylogenetic analyses were performed on partial fusion gene sequences of HMPV-positive patients.Results. HMPV-positive patients were detected in 2007-2008. The overall infection rate was 4%, while 57% of the patients were children. Sequence analysis demonstrated circulation of subgroup B viruses with predominance of lineage B2. Nucleotide sequence identity within lineage B1 was 98.8%–99.7% and higher than that in lineage B2 (94.3%–100%). Three new amino acid substitutions (T223N, R229K, and D280N) of lineage B2 were observed.Conclusion. HMPV is a major viral pathogen in the Egyptian population especially in children. During 2007-2008, predominantly HMPV B2 circulated in Upper Egypt. |
| Databáze: | OpenAIRE |
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