Transcriptional photoregulation of cell-type-preferred expression of maize rbcS-m3: 3' and 5' sequences are involved
| Autor: | Lawrence Bogorad, Jean-Frederic Viret, Yasser M. Mabrouk |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Oxygenase
Light Transcription Genetic Ribulose-Bisphosphate Carboxylase Molecular Sequence Data Restriction Mapping Biology Genes Plant Zea mays Restriction map Transcription (biology) Gene family RNA Messenger Promoter Regions Genetic Gene DNA Primers Repetitive Sequences Nucleic Acid Sequence Deletion Regulation of gene expression Multidisciplinary Base Sequence RuBisCO RNA Molecular biology Gene Expression Regulation biology.protein Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 91:8577-8581 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.91.18.8577 |
| Popis: | In the C4 plant maize, members of the rbcS gene family, encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase, are not expressed in mesophyll cells (MC) but are expressed strongly in the adjacent bundle sheath cells (BSC). Expression of genes in an in situ transient expression assay indicates that the photostimulated expression seen in BSC during the first 24 h that leaves of dark-grown seedlings are illuminated requires rbcS-m3 sequences lying between -211 bp and +434 bp of the transcription start site. Photoregulated partial suppression of rbcS-m3 expression in MC, on the other hand, requires gene sequences that lie between -907 bp and -445 bp together with sequences that lie between +720 and +957 bp within the 3' transcribed region of the gene. Suppression in MC occurs during the second 24-h period that dark-grown seedlings have been illuminated, but not during the first 24 h. The 3' +720- to +957-bp region is also effective in lowering MC expression when it is relocated to a position > 2 kbp upstream of the transcription start site. Thus, suppression of rbcS-m3 expression in MC has, at the least, a substantial transcriptional component. As reported earlier, a converse pattern of suppression in BSC and stimulation of expression in MC is seen in the control of cab-m1 in maize leaves. |
| Databáze: | OpenAIRE |
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