Astaxanthin suppresses scavenger receptor expression and matrix metalloproteinase activity in macrophages
| Autor: | Harumi Uto-Kondo, Yoshimi Kishimoto, Maki Iizuka, Hideaki Kurata, Hirohito Sone, Mariko Tani, Kazuo Kondo, Emi Saita |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
CD36 Antigens
CD36 Nitric Oxide Synthase Type II Medicine (miscellaneous) Xanthophylls Matrix metalloproteinase Antioxidants Cell Line chemistry.chemical_compound Astaxanthin Humans RNA Messenger Phosphorylation Scavenger receptor Receptor Nutrition and Dietetics biology Macrophages NF-kappa B Proteolytic enzymes Scavenger Receptors Class A Macrophage Activation Atherosclerosis Molecular biology Matrix Metalloproteinases Enzyme Activation Isoenzymes Nitric oxide synthase Gene Expression Regulation Biochemistry chemistry Cyclooxygenase 2 biology.protein Tumor necrosis factor alpha Inflammation Mediators Protein Processing Post-Translational |
| Zdroj: | European Journal of Nutrition. 49:119-126 |
| ISSN: | 1436-6215 1436-6207 |
| DOI: | 10.1007/s00394-009-0056-4 |
| Popis: | Astaxanthin is a red carotenoid pigment which has significant potential for antioxidant activity. The macrophages in atherosclerotic lesions, known as activated macrophages, express scavenger receptors responsible for the clearance of pathogenic lipoproteins. In addition, the expression and secretion of proteolytic enzymes, matrix metalloproteinases (MMPs), and pro-inflammatory cytokines are remarkably promoted in activated macrophages.In this study, we investigated the effects of astaxanthin on the expression of scavenger receptors, MMPs, and pro-inflammatory cytokines in macrophages.THP-1 macrophages were incubated with 5-10 microM astaxanthin for 24 h. The expression levels of scavenger receptors, MMPs, and pro-inflammatory cytokines were determined by Western blot analysis or real-time RT-PCR. The MMP-9 and -2 activities were examined by gelatin zymography and total MMP activity was measured by fluorometry.We found that astaxanthin remarkably decreased the class A scavenger receptor and CD36 expression in the protein and mRNA levels. Astaxanthin also reduced MMP-1, -2, -3, -9, -12, and -14 activity and expression. The mRNA expression of tumor necrosis factor-alpha, interleukin-1beta, interleukin-6, inducible nitric oxide synthase, and cyclooxygenase-2 were significantly suppressed by astaxanthin. Furthermore, astaxanthin inhibited the phosphorylation of nuclear factor-kappaB.These results indicate that astaxanthin has inhibitory effects on macrophage activation, such as scavenger receptors up-regulation, MMPs activation, and pro-inflammatory cytokines secretion. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink