Involvement of polyamines in epidermal growth factor (EGF), transforming growth factor (TGF)-α and -β1 action on culture of L6 and fetal bovine myoblasts
| Autor: | Tomasz Motyl, M. Kasterka, Arkadiusz Orzechowski, Boźena Interewicz, Katarzyna Grzelkowska, S. Blachowski |
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| Rok vydání: | 1994 |
| Předmět: |
Adenosylmethionine Decarboxylase
Eflornithine Spermidine Spermine Stimulation Biology Ornithine Decarboxylase Biochemistry Cell Line Ornithine decarboxylase chemistry.chemical_compound Transforming Growth Factor beta Epidermal growth factor Polyamines Animals Humans Cells Cultured Epidermal Growth Factor Muscles Transforming Growth Factor alpha Molecular biology Rats chemistry Protein Biosynthesis Cattle Signal transduction Polyamine Signal Transduction Transforming growth factor |
| Zdroj: | International Journal of Biochemistry. 26:891-897 |
| ISSN: | 0020-711X |
| Popis: | 1. 1. α-Difluoromethylornithine, an irreversible inhibitor of ornithine decarboxylase significantly abolished stimulation of protein synthesis evoked by EGF, TGF-α or -β 1 in L6 and fetal bovine myoblasts. 2. 2. The participation of polyamines in early events evoked by growth factors was shown by a significant stimulation of ornithine decarboxylase and Sdenosylmethionine decarboxylase activity as well as increased concentration of spermidine and spermine in L6 cells exposed to TGF-α and EGF. 3. 3. TGF-β 1 at a high concentration (1 ng/ml) increased protein synthesis in L6 myoblasts but inhibited it in fetal bovine myoblasts. Metabolic effects of TGF-β 1 in L6 cells was associated with an enhancement of decarboxylase activities, however there were no significant changes in cellular polyamine concentrations. Presented data suggest that polyamines are involved in the signal transduction pathway of EGF, TGF-α, and -β 1 in L6 and fetal bovine myoblasts. |
| Databáze: | OpenAIRE |
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