Skin extracellular matrix components accelerate the regenerative potential of Lin-cells
Autor: | Dovilė Žalalytė, Aida Vaitkuvienė, Lilija Kalėdienė, Genė Biziulevičienė, Giedrė Ramanauskaitė, Vytautas Kašėta |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
Basement membrane
General Immunology and Microbiology medicine.diagnostic_test Cell growth QH301-705.5 General Neuroscience proliferation Cell Cell migration in vitro lineage negative cells Matrix (biology) Biology migration General Biochemistry Genetics and Molecular Biology balb/c mouse Cell biology Flow cytometry Transplantation Extracellular matrix medicine.anatomical_structure medicine Biology (General) General Agricultural and Biological Sciences Lineage negative cells BALB/c mouse Proliferation Migration In vitro |
Zdroj: | Central European journal of biology, Warsaw : Versita, 2014, Vol. 9, no. 4, p. 367-373 Open Life Sciences, Vol 9, Iss 4, Pp 367-373 (2014) |
ISSN: | 1895-104X |
Popis: | Due to their unique properties, bone marrow-derived Lin− cells can be used to regenerate damaged tissues, including skin. The objective of our study was to determine the influence of the skin tissue-specific microenvironment on mouse Lin− cell proliferation and migration in vitro. Cells were analyzed for the expression of stem/progenitor surface markers by flow cytometry. Proliferation of MACS-purified cells in 3D cultures was investigated by WST-8 assay. Lin− cell migration was evaluated by in vitro scratch assay. The results obtained show that basement membrane matrix is more effective for Lin− cell proliferation in vitro. However, type I collagen matrix better enhances the re-epithelization process, that depends on the cell migratory properties. These studies are important for preparing cells to be used in transplantation. |
Databáze: | OpenAIRE |
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