Multiplex PCR assay specific for the multidrug-resistant strain W of Mycobacterium tuberculosis
| Autor: | Thomas M. Shinnick, J.L. Marden, W R Butler, Bonnie B. Plikaytis, Jack T. Crawford, Charles L. Woodley |
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| Rok vydání: | 1994 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Tuberculosis Rifabutin Molecular Sequence Data Antitubercular Agents New York Biology Polymerase Chain Reaction Disease Outbreaks Microbiology Mycobacterium tuberculosis Tuberculosis Multidrug-Resistant Multiplex polymerase chain reaction medicine Humans Ethambutol Retrospective Studies Cross Infection Base Sequence Strain (chemistry) Drug Resistance Microbial Amplicon bacterial infections and mycoses medicine.disease biology.organism_classification Virology Prisons DNA Transposable Elements Florida New York City Ethionamide Polymorphism Restriction Fragment Length Research Article medicine.drug |
| Zdroj: | Journal of Clinical Microbiology. 32:1542-1546 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | In 1991, a multidrug-resistant strain of Mycobacterium tuberculosis was isolated from eight people with tuberculosis at a state correctional facility in New York. This strain, which is designated strain W (IS6110 restriction fragment length polymorphism type 212072), was resistant to isoniazid, rifampin, ethambutol, streptomycin, kanamycin, ethionamide, and rifabutin. Since that outbreak, the W strain has been associated with outbreaks in five hospitals in the New York City area and is a continuing public health problem in the area. To be able to identify this strain rapidly, we developed a multiplex PCR assay which targets a direct repeat of IS6110 with a 556-bp intervening sequence (NTF-1). The amplification generates two amplicons from strain W, which indicate the presence and orientation of the NTF-1 sequence between the direct repeat of IS6110, and a third amplicon, which serves as an internal PCR control. The assay was evaluated with 193 isolates of M. tuberculosis, and all 48 strain W isolates among those 193 isolates were correctly identified. |
| Databáze: | OpenAIRE |
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