Molecular Biological Study of Anti-cancer Effects of Bee Venom Aqua-acupuncture
| Autor: | Chan-Yol Park, Jung-Chul Seo, Byoung-Choul Ahn, Do-Young Choi |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_treatment
lcsh:Medicine Biology complex mixtures chemistry.chemical_compound Gene expression medicine Transcriptional regulation molecular biology MTT assay Viability assay lcsh:Miscellaneous systems and treatments Gene Pharmacology anti-cancer effect Protease lcsh:R lcsh:RM1-950 lcsh:RZ409.7-999 Molecular biology lcsh:Therapeutics. Pharmacology Complementary and alternative medicine chemistry been venom Apoptosis Thymidine |
| Zdroj: | Journal of Pharmacopuncture, Vol 3, Iss 1, Pp 1-19 (2000) |
| ISSN: | 1226-4849 |
| DOI: | 10.3831/kpi.2000.3.1.001 |
| Popis: | To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability and apoptosis were analyzed using MTT assay, tryphan blue assay, [3H]thymidine release assay, flow cytometric analysis, and activity of caspase-3 protease activity assay. To explore whether anti-cancer effects of bee venom are associated with the transcriptional control of gene expression, quantitative RT-PCR analysis of apoptosis-related genes was performed. The obtained results are summarized as follows: 1. The MTT assay demonstrated that cell viability was decreased by bee venom in a dose-dependant manner. 2. Significant induction of apoptosis was identified using tryphan blue assay, [3H]thymidine release assay, and flow cytometric analysis of sub G1 fraction. 3. In analysis of caspase-3 protease activity, the activity had increased significantly, in a dose-dependant manner. 4. Quantitative RT-PCR analysis of the apoptosis-related genes showed that Bcl-2 and Bcl-XL were down-regulated whereas Bax was up-regulated by bee venom treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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